Please use this identifier to cite or link to this item: https://doi.org/10.1080/00016480701762490
DC FieldValue
dc.titleL-N-Acetylcysteine protects against radiation-induced apoptosis in a cochlear cell line
dc.contributor.authorLow, W.-K.
dc.contributor.authorSun, L.
dc.contributor.authorTan, M.G.K.
dc.contributor.authorChua, A.W.C.
dc.contributor.authorWang, D.-Y.
dc.date.accessioned2016-11-29T01:20:17Z
dc.date.available2016-11-29T01:20:17Z
dc.date.issued2008
dc.identifier.citationLow, W.-K., Sun, L., Tan, M.G.K., Chua, A.W.C., Wang, D.-Y. (2008). L-N-Acetylcysteine protects against radiation-induced apoptosis in a cochlear cell line. Acta Oto-Laryngologica 128 (4) : 440-445. ScholarBank@NUS Repository. https://doi.org/10.1080/00016480701762490
dc.identifier.issn00016489
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/131577
dc.description.abstractConclusion. L-N-Acetylcysteine (L-NAC) significantly reduced reactive oxygen species (ROS) generation and cochlear cell apoptosis after irradiation. The safe and effective use of L-NAC in reducing radiation-induced sensorineural hearing loss (SNHL) should be verified by further in vivo studies. Objectives. Radiation-induced SNHL is a common complication after radiotherapy of head and neck tumours. There is growing evidence to suggest that ROS play an important role in apoptotic cochlear cell death from ototoxicity, resulting in SNHL. The aim of this study was to evaluate the effectiveness of L-NAC, an antioxidant, on radiation-induced apoptosis in cochlear cells. Materials and methods. The OC-k3 cochlear cell line was studied after 0 and 20 Gy of γ-irradiation. Cell viability assay was performed using 3-[4,5-dimethylthiazol-2-yl]-2,5- diphenyltetrazolium bromide. Flow cytometry and TUNEL assay were done with and without the addition of 10 mmol/L of L-NAC. Intracellular generation of ROS was detected by 2′,7′-dichlorofluorescein diacetate, with comparisons made using fluorescence intensity. Results. L-NAC increased the viability of cells after irradiation. Generation of ROS was demonstrated at 1 h post-irradiation and was significantly reduced by L-NAC (p
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1080/00016480701762490
dc.sourceScopus
dc.subjectAntioxidant
dc.subjectDeafness
dc.subjectHair cell
dc.subjectHearing loss
dc.subjectHearing preservation
dc.subjectInner ear
dc.subjectIntratympanic
dc.subjectOtotoxicity
dc.subjectRadiation
dc.subjectRadiotherapy
dc.typeArticle
dc.contributor.departmentPHARMACOLOGY
dc.contributor.departmentOTOLARYNGOLOGY
dc.description.doi10.1080/00016480701762490
dc.description.sourcetitleActa Oto-Laryngologica
dc.description.volume128
dc.description.issue4
dc.description.page440-445
dc.description.codenAOLAA
dc.identifier.isiut000254394600019
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