Please use this identifier to cite or link to this item:
|Title:||L-N-Acetylcysteine protects against radiation-induced apoptosis in a cochlear cell line||Authors:||Low, W.-K.
|Issue Date:||2008||Citation:||Low, W.-K., Sun, L., Tan, M.G.K., Chua, A.W.C., Wang, D.-Y. (2008). L-N-Acetylcysteine protects against radiation-induced apoptosis in a cochlear cell line. Acta Oto-Laryngologica 128 (4) : 440-445. ScholarBank@NUS Repository. https://doi.org/10.1080/00016480701762490||Abstract:||Conclusion. L-N-Acetylcysteine (L-NAC) significantly reduced reactive oxygen species (ROS) generation and cochlear cell apoptosis after irradiation. The safe and effective use of L-NAC in reducing radiation-induced sensorineural hearing loss (SNHL) should be verified by further in vivo studies. Objectives. Radiation-induced SNHL is a common complication after radiotherapy of head and neck tumours. There is growing evidence to suggest that ROS play an important role in apoptotic cochlear cell death from ototoxicity, resulting in SNHL. The aim of this study was to evaluate the effectiveness of L-NAC, an antioxidant, on radiation-induced apoptosis in cochlear cells. Materials and methods. The OC-k3 cochlear cell line was studied after 0 and 20 Gy of γ-irradiation. Cell viability assay was performed using 3-[4,5-dimethylthiazol-2-yl]-2,5- diphenyltetrazolium bromide. Flow cytometry and TUNEL assay were done with and without the addition of 10 mmol/L of L-NAC. Intracellular generation of ROS was detected by 2′,7′-dichlorofluorescein diacetate, with comparisons made using fluorescence intensity. Results. L-NAC increased the viability of cells after irradiation. Generation of ROS was demonstrated at 1 h post-irradiation and was significantly reduced by L-NAC (p||Source Title:||Acta Oto-Laryngologica||URI:||http://scholarbank.nus.edu.sg/handle/10635/131577||ISSN:||00016489||DOI:||10.1080/00016480701762490|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Mar 2, 2021
WEB OF SCIENCETM
checked on Mar 2, 2021
checked on Feb 27, 2021
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.