Please use this identifier to cite or link to this item: https://doi.org/10.1634/stemcells.2007-0372
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dc.titlePleiotrophin enhances clonal growth and long-term expansion of human embryonic stem cells
dc.contributor.authorSOH BOON SENG
dc.contributor.authorChun, M.S.
dc.contributor.authorVallier, L.
dc.contributor.authorLi, P.
dc.contributor.authorChoong, C.
dc.contributor.authorBoon, H.Y.
dc.contributor.authorLim, E.H.
dc.contributor.authorPedersen, R.A.
dc.contributor.authorYang, H.H.
dc.contributor.authorRao, M.
dc.contributor.authorLima, B.
dc.date.accessioned2016-11-29T01:20:01Z
dc.date.available2016-11-29T01:20:01Z
dc.date.issued2007-12
dc.identifier.citationSOH BOON SENG, Chun, M.S., Vallier, L., Li, P., Choong, C., Boon, H.Y., Lim, E.H., Pedersen, R.A., Yang, H.H., Rao, M., Lima, B. (2007-12). Pleiotrophin enhances clonal growth and long-term expansion of human embryonic stem cells. Stem Cells 25 (12) : 3029-3037. ScholarBank@NUS Repository. https://doi.org/10.1634/stemcells.2007-0372
dc.identifier.issn10665099
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/131555
dc.description.abstractTo identify additional growth factors for optimizing propagation of human embryonic stem cells (hESCs), we mined publicly available data sets for the transcriptomes of murine and human ESCs and feeder cells, thereby generating a list of growth factors and complementary receptors. We identified the major pathways previously reported to be important, as well as several new ones. One pathway is the Pleiotrophin (PTN)-Pleiotrophin receptor (PTPRZ1) axis. Murine fibroblasts secrete Ptn, whereas hESCs expressed PTPRZ1, which is downregulated upon differentiation. Depletion of PTPRZ1 resulted in decreased colony formation and lower recovery of hESCs. Supplementation of chemically defined medium for feeder-free propagation of hESCs with PTN allowed higher recovery of hESCs without loss of pluripotency. PTN-PTPRZ1 functions here predominantly via an antiapoptotic effect mediated in part by the activation of Akt. These findings reveal the underlying importance of PTN in hESC survival and its usefulness in the clonal manipulation and large-scale propagation of hESCs. ©AlphaMed Press.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1634/stemcells.2007-0372
dc.sourceScopus
dc.subjectClonal efficiency and embryonic stem expansion
dc.subjectFeeder cells and human embryonic stem cells
dc.subjectGrowth factors and receptors
dc.subjectTranscriptome database
dc.typeArticle
dc.contributor.departmentMEDICINE
dc.contributor.departmentMICROBIOLOGY
dc.description.doi10.1634/stemcells.2007-0372
dc.description.sourcetitleStem Cells
dc.description.volume25
dc.description.issue12
dc.description.page3029-3037
dc.description.codenSTCEE
dc.identifier.isiut000251707200007
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