Please use this identifier to cite or link to this item:
|Title:||Monoclonal antibodies against the fusion peptide of hemagglutinin protect mice from lethal influenza A virus H5N1 infection||Authors:||Prabhu, N.
|Issue Date:||Mar-2009||Citation:||Prabhu, N., Prabakaran, M., Ho, H.-T., Velumani, S., Qiang, J., Goutama, M., Kwang, J. (2009-03). Monoclonal antibodies against the fusion peptide of hemagglutinin protect mice from lethal influenza A virus H5N1 infection. Journal of Virology 83 (6) : 2553-2562. ScholarBank@NUS Repository. https://doi.org/10.1128/JVI.02165-08||Abstract:||The HA2 glycopolypeptide (gp) is highly conserved in all influenza A virus strains, and it is known to play a major role in the fusion of the virus with the endosomal membrane in host cells during the course of viral infection. Vaccines and therapeutics targeting this HA2 gp could induce efficient broad-spectrum immunity against influenza A virus infections. So far, there have been no studies on the possible therapeutic effects of monoclonal antibodies (MAbs), specifically against the fusion peptide of hemagglutinin (HA), upon lethal infections with highly pathogenic avian influenza (HPAI) H5N1 virus. We have identified MAb 1C9, which binds to GLFGAIAGF, a part of the fusion peptide of the HA2 gp. We evaluated the efficacy of MAb 1C9 as a therapy for influenza A virus infections. This MAb, which inhibited cell fusion in vitro when administered passively, protected 100% of mice from challenge with five 50% mouse lethal doses of HPAI H5N1 influenza A viruses from two different clades. Furthermore, it caused earlier clearance of the virus from the lung. The influenza virus load was assessed in lung samples from mice challenged after pretreatment with MAb 1C9 (24 h prior to challenge) and from mice receiving early treatment (24 h after challenge). The study shows that MAb 1C9, which is specific to the antigenically conserved fusion peptide of HA2, can contribute to the cross-clade protection of mice infected with H5N1 virus and mediate more effective recovery from infection. Copyright © 2009, American Society for Microbiology.||Source Title:||Journal of Virology||URI:||http://scholarbank.nus.edu.sg/handle/10635/131552||ISSN:||0022538X||DOI:||10.1128/JVI.02165-08|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Mar 27, 2020
WEB OF SCIENCETM
checked on Mar 19, 2020
checked on Mar 27, 2020
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.