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https://doi.org/10.1016/j.vaccine.2004.09.015
Title: | Intragastric administration of Lactobacillus casei expressing transmissible gastroentritis coronavirus spike glycoprotein induced specific antibody production | Authors: | Ho, P.S. Kwang, J. Lee, Y.K. |
Keywords: | Coronavirus Oral vaccination Spike glycoprotein |
Issue Date: | 3-Feb-2005 | Citation: | Ho, P.S., Kwang, J., Lee, Y.K. (2005-02-03). Intragastric administration of Lactobacillus casei expressing transmissible gastroentritis coronavirus spike glycoprotein induced specific antibody production. Vaccine 23 (11) : 1335-1342. ScholarBank@NUS Repository. https://doi.org/10.1016/j.vaccine.2004.09.015 | Abstract: | Lactobacillus casei strain Shirota was selected as a bacterial carrier for the development of live mucosal vaccines against coronavirus. A 75 kDa fragment of transmissible gastroenteritis coronavirus (TGEV) spike glycoprotein S was used as the model coronavirus antigen. The S glycoprotein was cloned into a Lactobacillus/E. coli shuttle vector (pLP500) where expression and secretion of the glycoprotein S from the recombinant lactobacilli was detected via immunoblotting. Oral immunization of BALB/c mice with recombinant LcS that constitutively expresses the 75 kDa fragment of the glycoprotein S, induced both local mucosal and systemic immune responses against TGEV. Maximum titers of IgG (8.38 ± 0.19 ng/ml of serum) and IgA (64.82 ± 2.9 ng/ml of intestinal water) were attained 32 days post oral inturbation. The induced antibodies demonstrated neutralizing effects on TGEV infection. © 2004 Elsevier Ltd. All rights reserved. | Source Title: | Vaccine | URI: | http://scholarbank.nus.edu.sg/handle/10635/131546 | ISSN: | 0264410X | DOI: | 10.1016/j.vaccine.2004.09.015 |
Appears in Collections: | Staff Publications |
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