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Title: Identification of vaccine candidate antigens of an ESBL producing Klebsiella pneumoniae clinical strain by immunoproteome analysis
Authors: Kurupati, P.
Teh, B.K.
Kumarasinghe, G.
Poh, C.L. 
Keywords: Immunoblotting
Klebsiella pneumoniae
Outer membrane protein
Vaccine candidate
Issue Date: Feb-2006
Citation: Kurupati, P., Teh, B.K., Kumarasinghe, G., Poh, C.L. (2006-02). Identification of vaccine candidate antigens of an ESBL producing Klebsiella pneumoniae clinical strain by immunoproteome analysis. Proteomics 6 (3) : 836-844. ScholarBank@NUS Repository.
Abstract: Klebsiella pneumoniae is an opportunistic pathogen which causes pneumoniae, urinary tract infections and septicemia in immunocompromised patients. Hospital outbreaks of multidrug-resistant K. pneumoniae, especially those in neonatal wards, are often caused by strains producing the extended-spectrum-β- lactamases (ESBLs). An immunoproteome based approach was developed to identify candidate antigens of K. pneumoniae for vaccine development. Sera from patients with acute K. pneumoniae infections (n = 55) and a control group of sera from healthy individuals (n = 15) were analyzed for reactivity by Western blot against ESBL K. pneumoniae outer membrane proteins separated by 2-DE. Twenty highly immunogenic protein spots were identified by immunoproteomic analysis. The immunogenic proteins that are most frequently recognized by positive K. pneumoniae sera were OmpA, OmpK36, FepA, OmpK17, OmpW, Colicin I receptor protein and three novel proteins. Two of the vaccine candidate genes, OmpA (Struve et al. Microbiology 2003, 149, 167-176) and FepA (Lai, Y. C. et al. Infect Immun 2001, 69, 7140-7145), have recently been shown to be essential in colonization and infection in an in vivo mouse model. Hence, these two immunogenic proteins could serve as potential vaccine candidates. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.
Source Title: Proteomics
ISSN: 16159853
DOI: 10.1002/pmic.200500214
Appears in Collections:Staff Publications

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