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|Title:||Identification of H-specific determinants in flagellin of four Escherichia coli strains||Authors:||Seah, J.-N.
|Issue Date:||2000||Citation:||Seah, J.-N., Kwang, J. (2000). Identification of H-specific determinants in flagellin of four Escherichia coli strains. Archives of Microbiology 174 (1-2) : 28-34. ScholarBank@NUS Repository. https://doi.org/10.1007/s002030000167||Abstract:||The H serogroup of Escherichia coli is determined by the flagellar antigen, flagellin. Sequence analysis of the flagellin gene, fliC, reveals a central variable region and the highly conserved N- and C-termini. This variable region has been shown to encode both H-specific and cross-reactive epitopes. Using polyclonal antibodies, we mapped the linear H-specific determinants in flagellin from four E. coli serotypes O157:H10, O138:H14, O157:H42 and O157:H43. The specificity of all potential fragments was verified with 52 ECRC (Escherichia coli Reference Center) H-specific antisera. Our results indicated that: (a) a specific determinant of H10 flagellin (1263 bp long) maps to the region covering amino acid residues 305-331; (b) a specific determinant of H14 flagellin (1653 bp long) maps to the region covering amino acid residues 430-461; (c) a specific determinant of H42 flagellin (1281 bp long) maps to a region covering amino acid residues 171-201; and (d) a specific determinant of H43 flagellin (1506 bp long) maps to a region covering amino acid residues 200-260.||Source Title:||Archives of Microbiology||URI:||http://scholarbank.nus.edu.sg/handle/10635/131543||ISSN:||03028933||DOI:||10.1007/s002030000167|
|Appears in Collections:||Staff Publications|
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