Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/131487
DC FieldValue
dc.titleExpression of the dengue virus structural proteins in Pichia pastoris leads to the generation of virus-like particles
dc.contributor.authorSugrue, R.J.
dc.contributor.authorFu, J.
dc.contributor.authorHowe, J.
dc.contributor.authorChan, Y.-C.
dc.date.accessioned2016-11-28T10:20:49Z
dc.date.available2016-11-28T10:20:49Z
dc.date.issued1997-08
dc.identifier.citationSugrue, R.J., Fu, J., Howe, J., Chan, Y.-C. (1997-08). Expression of the dengue virus structural proteins in Pichia pastoris leads to the generation of virus-like particles. Journal of General Virology 78 (8) : 1861-1866. ScholarBank@NUS Repository.
dc.identifier.issn00221317
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/131487
dc.description.abstractWe have expressed cDNA encoding the dengue virus structural proteins in Pichia pastoris by chromosomal integration of an expression cassette containing the dengue virus structural genes (CprME). The yeast recombinant E protein migrated during SDS-PAGE as a 65 kDa protein when analysed by Western blotting and radioimmunoprecipitation, which is the expected molecular mass for correctly processed and glycosylated E protein. Treatment with endoglycosidases showed that the recombinant E protein was modified by the addition of short mannose chains. The E protein migrated with a buoyant density of 1.13 g/cm3 when analysed using sucrose density gradient centrifugation. Spherical structures with an average diameter of 30 nm, whose morphology resembles dengue virions, were observed in the purified fractions using transmission electron microscopy. Furthermore, the virus-like particles were immunogenic in animals and were able to induce neutralizing antibodies. This is the first report that expression of the structural genes of a flavivirus in yeast is able to generate particulate structures that resemble virions.
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentINSTITUTE OF MOLECULAR & CELL BIOLOGY
dc.contributor.departmentMICROBIOLOGY
dc.description.sourcetitleJournal of General Virology
dc.description.volume78
dc.description.issue8
dc.description.page1861-1866
dc.description.codenJGVIA
dc.identifier.isiutNOT_IN_WOS
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

Page view(s)

23
checked on Jul 12, 2019

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.