Please use this identifier to cite or link to this item: https://doi.org/10.1186/1743-422X-5-151
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dc.titleIdentification and characterization of a new E3 ubiquitin ligase in white spot syndrome virus involved in virus latency
dc.contributor.authorHe, F.
dc.contributor.authorKwang, J.
dc.date.accessioned2016-11-28T10:20:35Z
dc.date.available2016-11-28T10:20:35Z
dc.date.issued2008
dc.identifier.citationHe, F., Kwang, J. (2008). Identification and characterization of a new E3 ubiquitin ligase in white spot syndrome virus involved in virus latency. Virology Journal 5 : -. ScholarBank@NUS Repository. https://doi.org/10.1186/1743-422X-5-151
dc.identifier.issn1743422X
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/131467
dc.description.abstractWhite spot syndrome virus (WSSV) is one major pathogen in shrimp aquaculture. WSSV ORF403 is predicted to encode a protein of 641 amino acids, which contains a C3H2C2 RING structure. In the presence of an E2 conjugating enzyme from shrimp, WSSV403 can ubiquitinate itself in vitro, indicating it can function as a viral E3 ligase. Besides, WSSV403 E3 ligase can be activated by a series of E2 variants. Based on RT-PCR and Real time PCR, we detected transcription of WSSV403 in the commercial specific-pathogen-free (SPF) shrimp, suggesting its role as a latency-associated gene. Identified in yeast two-hybrid screening and verified by pull-down assays, WSSV403 is able to bind to a shrimp protein phosphatase (PPs), which was characterized before as an interaction partner for another latent protein WSSV427. Our studies suggest that WSSV403 is a regulator of latency state of WSSV by virtue of its E3 ligase function. © 2008 He and Kwang; licensee BioMed Central Ltd.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1186/1743-422X-5-151
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentCHEMISTRY
dc.contributor.departmentMICROBIOLOGY
dc.description.doi10.1186/1743-422X-5-151
dc.description.sourcetitleVirology Journal
dc.description.volume5
dc.description.page-
dc.identifier.isiut000262529100001
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