Please use this identifier to cite or link to this item:
|Title:||Inhibition of porcine circovirus type 2 replication in mice by RNA interference||Authors:||Liu, J.
Porcine circovirus type 2 (PCV2)
RNA interference (RNAi)
|Issue Date:||10-Apr-2006||Citation:||Liu, J., Chen, I., Chua, H., Du, Q., Kwang, J. (2006-04-10). Inhibition of porcine circovirus type 2 replication in mice by RNA interference. Virology 347 (2) : 422-433. ScholarBank@NUS Repository. https://doi.org/10.1016/j.virol.2005.12.006||Abstract:||Porcine circovirus type 2 (PCV2) is the primary causative agent of an emerging swine disease, postweaning multisystemic wasting syndrome (PMWS) for which no antiviral treatment is available. To exploit the possibility of using RNA interference (RNAi) as a therapeutic approach against the disease, plasmid-borne short hairpin RNAs (shRNAs) were generated to target the PCV2 genome. Transfection of these shRNAs into cultured PK15 cells caused a significant reduction in viral RNA production that was accompanied by inhibiting viral DNA replication and protein synthesis in infected cells. The effect was further tested in vivo in a mouse model that has been developed for PCV2 infection. Mice injected with shRNA before PCV2 infection showed substantially decreased microscopic lesions in inguinal lymph nodes compared to controls. In situ hybridization and immunohistochemical analyses showed that shRNA caused a significant inhibition in the level of viral DNA and protein synthesis detected in the lymph nodes of the treated mice relative to the controls. Taken together, these results indicate that shRNAs are capable of inhibiting PCV2 infection in vitro as well as in vivo and thus may constitute an effective therapeutic strategy for PCV2 infection. © 2005 Elsevier Inc. All rights reserved.||Source Title:||Virology||URI:||http://scholarbank.nus.edu.sg/handle/10635/131466||ISSN:||00426822||DOI:||10.1016/j.virol.2005.12.006|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Mar 4, 2021
WEB OF SCIENCETM
checked on Mar 4, 2021
checked on Feb 27, 2021
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.