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https://doi.org/10.1128/JCM.42.4.1570-1576.2004
Title: | Immunological Characterization of the Spike Protein of the Severe Acute Respiratory Syndrome Coronavirus | Authors: | Lu, L. Manopo, I. Leung, B.P. Chng, H.H. Ling, A.E. Chee, L.L. Ooi, E.E. Chan, S.-W. Kwang, J. |
Issue Date: | Apr-2004 | Citation: | Lu, L., Manopo, I., Leung, B.P., Chng, H.H., Ling, A.E., Chee, L.L., Ooi, E.E., Chan, S.-W., Kwang, J. (2004-04). Immunological Characterization of the Spike Protein of the Severe Acute Respiratory Syndrome Coronavirus. Journal of Clinical Microbiology 42 (4) : 1570-1576. ScholarBank@NUS Repository. https://doi.org/10.1128/JCM.42.4.1570-1576.2004 | Abstract: | Severe acute respiratory syndrome (SARS) is a novel infectious disease caused by the SARS-associated coronavirus (SARS-CoV). There are four major structural proteins in the SARS-CoV, including the nucleocapsid, spike, membrane, and small envelope proteins. In this study, two sets of truncated fragments of spike protein were generated, the first were approximately 210-bp nonoverlapping fragments and the second were overlapping segments of 750 to 900 bp. From these 23 fragments, we identified a fragment of 259 amino acids (amino acids 441 to 700) that is a major immunodominant epitope. This fragment was highly expressed, and the purified fragment C could detect all 33 SARS patient serum samples tested, collected from 7 to 60 days after the onset of fever, but had no reactivity with all 66 healthy human serum samples tested. Thus, fragment C of spike protein was identified as an immunodominant antigen and could be used for serological detection of SARS-CoV infection. | Source Title: | Journal of Clinical Microbiology | URI: | http://scholarbank.nus.edu.sg/handle/10635/131408 | ISSN: | 00951137 | DOI: | 10.1128/JCM.42.4.1570-1576.2004 |
Appears in Collections: | Staff Publications |
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