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Title: Immunological Characterization of the Spike Protein of the Severe Acute Respiratory Syndrome Coronavirus
Authors: Lu, L.
Manopo, I.
Leung, B.P. 
Chng, H.H.
Ling, A.E.
Chee, L.L. 
Ooi, E.E. 
Chan, S.-W. 
Kwang, J. 
Issue Date: Apr-2004
Citation: Lu, L., Manopo, I., Leung, B.P., Chng, H.H., Ling, A.E., Chee, L.L., Ooi, E.E., Chan, S.-W., Kwang, J. (2004-04). Immunological Characterization of the Spike Protein of the Severe Acute Respiratory Syndrome Coronavirus. Journal of Clinical Microbiology 42 (4) : 1570-1576. ScholarBank@NUS Repository.
Abstract: Severe acute respiratory syndrome (SARS) is a novel infectious disease caused by the SARS-associated coronavirus (SARS-CoV). There are four major structural proteins in the SARS-CoV, including the nucleocapsid, spike, membrane, and small envelope proteins. In this study, two sets of truncated fragments of spike protein were generated, the first were approximately 210-bp nonoverlapping fragments and the second were overlapping segments of 750 to 900 bp. From these 23 fragments, we identified a fragment of 259 amino acids (amino acids 441 to 700) that is a major immunodominant epitope. This fragment was highly expressed, and the purified fragment C could detect all 33 SARS patient serum samples tested, collected from 7 to 60 days after the onset of fever, but had no reactivity with all 66 healthy human serum samples tested. Thus, fragment C of spike protein was identified as an immunodominant antigen and could be used for serological detection of SARS-CoV infection.
Source Title: Journal of Clinical Microbiology
ISSN: 00951137
DOI: 10.1128/JCM.42.4.1570-1576.2004
Appears in Collections:Staff Publications

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