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Title: Interaction of kunjin virus with octyl-D-glucoside extracted Vero cell plasma membrane
Authors: Sankaran, D. 
Lau, L.C.L. 
Ng, M.L. 
Keywords: flavivirus
octyl-D-glucoside extract
Issue Date: Jan-1997
Citation: Sankaran, D., Lau, L.C.L., Ng, M.L. (1997-01). Interaction of kunjin virus with octyl-D-glucoside extracted Vero cell plasma membrane. Journal of Virological Methods 63 (1-2) : 167-173. ScholarBank@NUS Repository.
Abstract: Initial experiments using whole cells have shown that there were specific and saturable interactions between kunjin (KUN) virus and receptor molecules on the Vero cell surfaces. Solubilisation of Vero cell plasma membranes with octyl-D-glucoside (OG) yielded an extract which also interacted specifically with KUN virus. This was proven using electron microscopy. When the virus-OG-extract complex was exposed onto Vero cell monolayers, no KUN virus was observed to enter into the whole cells. This would imply that there was virus-receptor interaction with the OG-extract leaving no free virus to attach to the whole cells. The attachment kinetics of KUN virus was studied further using the Scatchard analysis which indicated the involvement of more than one interactive macromolecule in the attachment event.
Source Title: Journal of Virological Methods
ISSN: 01660934
Appears in Collections:Staff Publications

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