Please use this identifier to cite or link to this item: https://doi.org/10.1006/plas.1997.1331
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dc.titleIS1491 from Pseudomonas alcaligenes NCIB 9867: Characterization and distribution among Pseudomonas species
dc.contributor.authorYeo, C.C.
dc.contributor.authorWong, D.T.S.
dc.contributor.authorPoh, C.L.
dc.date.accessioned2016-11-28T10:18:05Z
dc.date.available2016-11-28T10:18:05Z
dc.date.issued1998-05
dc.identifier.citationYeo, C.C., Wong, D.T.S., Poh, C.L. (1998-05). IS1491 from Pseudomonas alcaligenes NCIB 9867: Characterization and distribution among Pseudomonas species. Plasmid 39 (3) : 187-195. ScholarBank@NUS Repository. https://doi.org/10.1006/plas.1997.1331
dc.identifier.issn0147619X
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/131258
dc.description.abstractA new insertion sequence, IS1491, has been cloned and sequenced. The 2489-bp IS1491 was isolated from a Pseudomonas alcaligenes NCIB 9867 (strain P25X) 4.8-kb PstI chromosomal fragment. IS1491 is flanked by an imperfect inverted repeat of 23 bp and carries two overlapping open reading frames, ORF1 and ORF2. Both ORF1 and ORF2 displayed homology to the IstA-like and IstB-like transposases encoded by the IS21 family of insertion sequences, which include two IS elements previously isolated from P. alcaligenes P25X, IS1474, and IS1475 (Yeo, C. C., and Poh, C. L. (1997). FEMS Microbiol. Lett. 149, 257-263). Transposition assays showed that IS1491 transposed at a frequency of approximately 1.4 x 10-6. Transposition of IS1491 into the target pRK415 replicon was observed but when ORF2 was disrupted, a fusion between the donor and target replicons was detected. IS1491-like sequences were detected in total DNA of Pseudomonas putida NCIB 9869 (strain P35X), Pseudomonas aeruginosa, Pseudomonas stutzeri, Pseudomonas syringae, Pseudomonas mendocina, Comomonas acidovorans, and Comomonas testosteroni by hybridization with IS1491 DNA.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1006/plas.1997.1331
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentMICROBIOLOGY
dc.description.doi10.1006/plas.1997.1331
dc.description.sourcetitlePlasmid
dc.description.volume39
dc.description.issue3
dc.description.page187-195
dc.description.codenPLSMD
dc.identifier.isiut000073595000003
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