Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/131256
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dc.titleInvolvement of the heparan sulphate-binding proteins of Helicobacter pylori in its adherence to HeLa S3 and Kato III cell lines
dc.contributor.authorGuzman-Murillo, M.A.
dc.contributor.authorRuiz-Bustos, E.
dc.contributor.authorHo, B.
dc.contributor.authorAscencio, F.
dc.date.accessioned2016-11-28T10:18:04Z
dc.date.available2016-11-28T10:18:04Z
dc.date.issued2001
dc.identifier.citationGuzman-Murillo, M.A., Ruiz-Bustos, E., Ho, B., Ascencio, F. (2001). Involvement of the heparan sulphate-binding proteins of Helicobacter pylori in its adherence to HeLa S3 and Kato III cell lines. Journal of Medical Microbiology 50 (4) : 320-329. ScholarBank@NUS Repository.
dc.identifier.issn00222615
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/131256
dc.description.abstractTo determine whether Helicobacter pylori heparan sulphate-binding proteins (HSBPs) are involved in the adherence of H. pylori to HeLa and Kato III cells, monolayers were pre-incubated with various preparations and concentrations of H. pylori HSBPs at 37°C, washed and then challenged with bacteria. HSBPs did not prevent but enhanced H. pylori adherence. However, challenging cultured cells with H. pylori previously incubated with rabbit anti-HSBP IgG resulted in significant inhibition of bacterial adherence. These data demonstrate that the extracellular HSBP plays an important role in promoting H. pylori attachment to Kato III and HeLa S3 cells, that adhesion of H. pylori to Kato III and HeLa S3 cells is promoted by the presence of the 71.5-kDa extracellular HSBP and that rabbit polyclonal antibodies against this HSBP can inhibit adhesion of H. pylori to the cultured cell lines and detach cell-bound H. pylori.
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentMICROBIOLOGY
dc.description.sourcetitleJournal of Medical Microbiology
dc.description.volume50
dc.description.issue4
dc.description.page320-329
dc.description.codenJMMIA
dc.identifier.isiutNOT_IN_WOS
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