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|Title:||Identification and cloning of a novel heterogeneous nuclear ribonucleoprotein C-like protein that functions as a transcriptional activator of the hepatitis B virus enhancer II||Authors:||Tay, N.
|Issue Date:||1992||Citation:||Tay, N., Chan, S.-H., Ren, E.-C. (1992). Identification and cloning of a novel heterogeneous nuclear ribonucleoprotein C-like protein that functions as a transcriptional activator of the hepatitis B virus enhancer II. Journal of Virology 66 (12) : 6841-6848. ScholarBank@NUS Repository.||Abstract:||Liver specificity of hepatitis B virus (HBV) replication has been attributed to the action of its second enhancer (EII). We report here the characterization of EII and the subsequent isolation of a novel liver- specific DNA-binding protein which binds to and activates EII. The cDNA clone of the protein, designated E2BP, was isolated from a λgt11 expression library constructed from the hepatoma cell line HuH-6 which was screened with a binding site probe derived from EII. Sequence analysis of E2BP revealed 86.6% homology with a rat heterogeneous nuclear ribonucleoprotein C protein sequence, while conformational studies suggest a helix-loop-helix motif as a DNA-binding site. Cloned E2BP expressed in human fibroblasts by transient transfection displayed EII binding and activating characteristics similar to those of native E2BP in hepatocytes.||Source Title:||Journal of Virology||URI:||http://scholarbank.nus.edu.sg/handle/10635/131249||ISSN:||0022538X|
|Appears in Collections:||Staff Publications|
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