Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/131200
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dc.titleIgG1 subclass dominates autoimmune response to tyrosine phosphatase-like molecule IA-2 in Chinese type 1 diabetes patients
dc.contributor.authorNg, W.Y.
dc.contributor.authorLui, K.F.
dc.contributor.authorCheah, J.S.
dc.contributor.authorThai, A.C.
dc.date.accessioned2016-11-28T10:17:23Z
dc.date.available2016-11-28T10:17:23Z
dc.date.issued2002-10-01
dc.identifier.citationNg, W.Y., Lui, K.F., Cheah, J.S., Thai, A.C. (2002-10-01). IgG1 subclass dominates autoimmune response to tyrosine phosphatase-like molecule IA-2 in Chinese type 1 diabetes patients. Hormone and Metabolic Research 34 (10) : 596-600. ScholarBank@NUS Repository.
dc.identifier.issn00185043
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/131200
dc.description.abstractBackground and Aims: Islet autoantibodies are known markers for type 1 diabetes with an immune-mediated basis; their isotype or subclass profiles may also provide clues to changes in immune response during disease or after intervention. For ICAs and GADab, the IgG1 subclass consistently dominates in recent-onset disease. The aims of our study were to determine the isotype patterns for IA-2ab in Asian Chinese patients with autoimmune diabetes. Materiais anal Methods: From an initial screening of over 400 diabetes patients, 40 subjects (mean age 22.2 ± 15.8 years) with IA-2ab were enrolled for this study. IA-2ab was detected by radioimmunoassay of [35S]-labelled recombinant human IA-2ic(605-979). Of them, 31 (median age 15 years, range 2-57 years; 16 children) had clinical type 1 diabetes (that is, they required insulin at onset or within 1 year) with the majority having been recently diagnosed (< 1 year). The other 9 patients had clinical type 2 diabetes phenotype. Results: IA-2ab IgG subclasses determined with monospecific secondary antibodies showed that both type 1 diabetic adults and children had similarly non-restricted isotype patterns with a strong presence of IgG1-IA-2ab. The rank order was IgG1 > 3 > 2 > 4; 15 subjects had detectable IgG4-1A-2ab. Clonality of immune response determined with kappa/lambda chain-specific antibodies also showed a non-restricted pattern. Patients aged 38.2 ± 15.2 years with type 2 diabetes had broad patterns of isotypes - IgG1/3 was detected more frequently (n = 8) than IgG2/4 (n = 5). Of three patients on insulin treatment, one was also positive for GADab. The remaining 6 patients were on oral hypoglycaemic treatment, IA-2ab in type 2 diabetes showed a low titre compared to type 1 diabetes. Conclusions: Isotype responses to IA-2 had a strong IgG1 presence, similar to ICAs and GADab. With IgG3 subclass representation, a predominant Th1 milieu in the systemic environment is likely. There is no suggestion of differences in immune response to IA-2 between adults and children with type 1 diabetes.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1055/s-2002-35422
dc.sourceScopus
dc.subjectIA-2 Antibodies
dc.subjectImmune-Mediated Diabetes
dc.subjectIsotypes
dc.typeArticle
dc.contributor.departmentMEDICINE
dc.description.sourcetitleHormone and Metabolic Research
dc.description.volume34
dc.description.issue10
dc.description.page596-600
dc.description.codenHMMRA
dc.identifier.isiut000179373400010
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