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Title: IgG1 subclass dominates autoimmune response to tyrosine phosphatase-like molecule IA-2 in Chinese type 1 diabetes patients
Authors: Ng, W.Y. 
Lui, K.F.
Cheah, J.S. 
Thai, A.C. 
Keywords: IA-2 Antibodies
Immune-Mediated Diabetes
Issue Date: 1-Oct-2002
Citation: Ng, W.Y., Lui, K.F., Cheah, J.S., Thai, A.C. (2002-10-01). IgG1 subclass dominates autoimmune response to tyrosine phosphatase-like molecule IA-2 in Chinese type 1 diabetes patients. Hormone and Metabolic Research 34 (10) : 596-600. ScholarBank@NUS Repository.
Abstract: Background and Aims: Islet autoantibodies are known markers for type 1 diabetes with an immune-mediated basis; their isotype or subclass profiles may also provide clues to changes in immune response during disease or after intervention. For ICAs and GADab, the IgG1 subclass consistently dominates in recent-onset disease. The aims of our study were to determine the isotype patterns for IA-2ab in Asian Chinese patients with autoimmune diabetes. Materiais anal Methods: From an initial screening of over 400 diabetes patients, 40 subjects (mean age 22.2 ± 15.8 years) with IA-2ab were enrolled for this study. IA-2ab was detected by radioimmunoassay of [35S]-labelled recombinant human IA-2ic(605-979). Of them, 31 (median age 15 years, range 2-57 years; 16 children) had clinical type 1 diabetes (that is, they required insulin at onset or within 1 year) with the majority having been recently diagnosed (< 1 year). The other 9 patients had clinical type 2 diabetes phenotype. Results: IA-2ab IgG subclasses determined with monospecific secondary antibodies showed that both type 1 diabetic adults and children had similarly non-restricted isotype patterns with a strong presence of IgG1-IA-2ab. The rank order was IgG1 > 3 > 2 > 4; 15 subjects had detectable IgG4-1A-2ab. Clonality of immune response determined with kappa/lambda chain-specific antibodies also showed a non-restricted pattern. Patients aged 38.2 ± 15.2 years with type 2 diabetes had broad patterns of isotypes - IgG1/3 was detected more frequently (n = 8) than IgG2/4 (n = 5). Of three patients on insulin treatment, one was also positive for GADab. The remaining 6 patients were on oral hypoglycaemic treatment, IA-2ab in type 2 diabetes showed a low titre compared to type 1 diabetes. Conclusions: Isotype responses to IA-2 had a strong IgG1 presence, similar to ICAs and GADab. With IgG3 subclass representation, a predominant Th1 milieu in the systemic environment is likely. There is no suggestion of differences in immune response to IA-2 between adults and children with type 1 diabetes.
Source Title: Hormone and Metabolic Research
ISSN: 00185043
Appears in Collections:Staff Publications

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