Please use this identifier to cite or link to this item:
|Title:||Hepatitis C virus non-structural protein NS3 interacts with LMP7, a component of the immunoproteasome, and affects its proteasome activity||Authors:||Khu, Y.-L.
|Keywords:||Hepatitis C virus (HCV)
Low-molecular-mass protein 7 (LMP7)
Non-structural protein NS3
|Issue Date:||1-Dec-2004||Citation:||Khu, Y.-L., Tan, Y.-J., Lim, S.G., Hong, W., Goh, P.-Y. (2004-12-01). Hepatitis C virus non-structural protein NS3 interacts with LMP7, a component of the immunoproteasome, and affects its proteasome activity. Biochemical Journal 384 (2) : 401-409. ScholarBank@NUS Repository. https://doi.org/10.1042/BJ20040858||Abstract:||NS3, a non-structural protein of the HCV (hepatitis C virus), contains a protease and a helicase domain and plays essential roles in the processing of the viral polyprotein, viral RNA replication and translation. LMP7 (low-molecular-mass protein 7), a component of the immunoproteasome, was identified as an NS3-binding protein from yeast two-hybrid screens, and this interaction was confirmed by in vitro binding and co-immunoprecipitation analysis. The minimal domain of interaction was defined to be between the pro-sequence region of LMP7 (amino acids 1-40) and the protease domain of NS3. To elucidate the biological importance of this interaction, we studied the effect of this interaction on NS3 protease activity and on LMP7 immunoproteasome activity. Recombinant LMP7 did not have any effect on NS3 protease activity in vitro. The peptidase activities of LMP7 immunoproteasomes, however, were markedly reduced when tested in a stable cell line containing a HCV subgenomic replicon. The down-regulation of proteasome peptidase activities could interfere with the processing of viral antigens for presentation by MHC class I molecules, and may thus protect HCV from host immune surveillance mechanisms to allow persistent infection by the virus.||Source Title:||Biochemical Journal||URI:||http://scholarbank.nus.edu.sg/handle/10635/131194||ISSN:||02646021||DOI:||10.1042/BJ20040858|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Aug 5, 2020
WEB OF SCIENCETM
checked on Jul 29, 2020
checked on Jul 31, 2020
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.