Please use this identifier to cite or link to this item:
|Title:||Fertilization and development of mouse oocytes injected with membrane-damaged spermatozoa||Authors:||Ahmadi, A.H.
Single sperm curling (SSC) test
|Issue Date:||1997||Citation:||Ahmadi, A.H., Ng, S.-C. (1997). Fertilization and development of mouse oocytes injected with membrane-damaged spermatozoa. Human Reproduction 12 (12) : 2797-2801. ScholarBank@NUS Repository. https://doi.org/10.1093/humrep/12.12.2797||Abstract:||The objective of this study was to investigate the influence of sperm plasma membrane on fertilization and development in a mouse model. The sperm plasma membrane was destroyed by exposure to Triton X-100 prior to intracytoplasmic sperm injection (ICSI). A single sperm curling (SSC) test was used to evaluate cell viability. The fertilization rates of oocytes obtained following ICSI of membrane-damaged sperm was not significantly higher than that of the control group (62.4 versus 59.6%). Rates of development to blastocyst were also not significantly different (51.7 and 50%). Inner cell mass (ICM) and total embryo cell numbers in the two groups were not statistically different (16 ± 3.7 versus 14.73 ± 3.35 and 45.8 ± 12.5 versus 39.5 ± 12 respectively). There were no differences in the implantation and live fetus rates between the two groups after transfer to pseudopregnant mice (61.5 and 35.9% respectively for the membrane-damaged group and 53.5 and 31.4% for the intact group). In conclusion, the present study clearly shows that destruction of the sperm plasma membrane does not affect fertilization and further development following injection of membrane-damaged spermatozoa into mouse oocytes. Fertilization and development can be achieved by dead spermatozoa at an early stage of necrosis when only the plasma membrane has been damaged.||Source Title:||Human Reproduction||URI:||http://scholarbank.nus.edu.sg/handle/10635/131028||ISSN:||02681161||DOI:||10.1093/humrep/12.12.2797|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Dec 11, 2019
WEB OF SCIENCETM
checked on Dec 3, 2019
checked on Dec 13, 2019
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.