Please use this identifier to cite or link to this item:
Title: Isolation and characterization of the mitochondrion-like organelle from Blastocystis hominis
Authors: Nasirudeen, A.M.A. 
Tan, K.S.W.
Keywords: Blastocystis
Mitochondrial transmembrane potential
Rhodamine 123
Issue Date: Jul-2004
Citation: Nasirudeen, A.M.A., Tan, K.S.W. (2004-07). Isolation and characterization of the mitochondrion-like organelle from Blastocystis hominis. Journal of Microbiological Methods 58 (1) : 101-109. ScholarBank@NUS Repository.
Abstract: Blastocystis hominis in an unusual protozoan parasite of the human intestinal tract. Previous studies have described the presence of mitochondrial-like structures despite the anaerobic nature of the organism. In this study, we describe a simple and rapid technique to isolate and characterize mitochondrion-like organelles (MLO) from B. hominis. The parasite was disrupted using glass beads and the MLO were collected and purified using a sucrose gradient. Negative staining and transmission electron microscopy of the isolated organelles showed mitochondrial-like structures. B. hominis cells were stained with rhodamine 123 and MitoLight™ to show the presence of transmembrane potential of the MLO. DAPI staining of the cells suggested the presence of DNA in the MLO. Though brief reports have been made in literature, this study is the first to describe a technique for the isolation of the MLO from this organism. Using this technique of isolation, major metabolic functions of the organelle, their associated macromolecules and intra-mitochondrial location can be extensively studied. The role of MLO in this anaerobic protozoan can be widely investigated using this protocol. © 2004 Elsevier B.V. All rights reserved.
Source Title: Journal of Microbiological Methods
ISSN: 01677012
DOI: 10.1016/j.mimet.2004.03.008
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.


checked on Nov 28, 2022


checked on Nov 28, 2022

Page view(s)

checked on Dec 1, 2022

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.