Please use this identifier to cite or link to this item: https://doi.org/10.1002/jmv.10393
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dc.titleHemagglutinin-neuraminidase sequence and phylogenetic analyses of mumps virus isolates from a vaccinated population in Singapore
dc.contributor.authorLim, C.S.
dc.contributor.authorChan, K.P.
dc.contributor.authorGoh, K.T.
dc.contributor.authorChow, V.T.K.
dc.date.accessioned2016-11-28T10:14:49Z
dc.date.available2016-11-28T10:14:49Z
dc.date.issued2003-06-01
dc.identifier.citationLim, C.S., Chan, K.P., Goh, K.T., Chow, V.T.K. (2003-06-01). Hemagglutinin-neuraminidase sequence and phylogenetic analyses of mumps virus isolates from a vaccinated population in Singapore. Journal of Medical Virology 70 (2) : 287-292. ScholarBank@NUS Repository. https://doi.org/10.1002/jmv.10393
dc.identifier.issn01466615
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/130975
dc.description.abstractDuring 1999-2000, a sustained mumps outbreak in the highly vaccinated population in Singapore was attributed to vaccine failure associated with the Rubini vaccine strain. To explain this phenomenon, the complete nucleotide and amino acid sequences of the hemagglutinin-neuraminidase (HN) gene of eight mumps virus isolates from patients with parotitis in Singapore were determined and compared with those of known vaccine strains. Phylogenetic trees constructed on the basis of HN nucleotide and amino acid sequences showed that the Singapore mumps virus isolates were more closely related to the Urabe strain and belonged to a different cluster from the Rubini and Jeryl-Lynn strains. The Rubini vaccine showed only ∼93% nucleotide and ∼96% amino acid sequence similarity to Urabe and Singapore isolates. Compared with the vaccine strains, six of the eight isolates lacked the extracellular glycosylation site at residues 400-402. Other significant amino acid disparities (e.g., at residue 354) may also affect the antigenic properties of the HN protein. These findings suggest that the evolution and adaptation of the currently circulating mumps virus strains in the community has led to the emergence of genetically distinct viral strains. The low vaccine efficacy of the Rubini strain represents a major reason for the recent mumps resurgence and failure of mumps immunization in Singapore. © 2003 Wiley-Liss, Inc.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1002/jmv.10393
dc.sourceScopus
dc.subjectHN gene
dc.subjectMumps virus
dc.subjectPhylogeny
dc.subjectRubini vaccine failure
dc.subjectSingapore
dc.typeArticle
dc.contributor.departmentMICROBIOLOGY
dc.description.doi10.1002/jmv.10393
dc.description.sourcetitleJournal of Medical Virology
dc.description.volume70
dc.description.issue2
dc.description.page287-292
dc.description.codenJMVID
dc.identifier.isiut000182564000018
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