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Title: Commissural axon pathfinding at intermediate targets in the zebrafish forebrain
Keywords: axon guidance, commissure, zebrafish, habenula, electroporation, Esrom
Issue Date: 6-Dec-2007
Citation: SHELTON MICHAEL HENDRICKS (2007-12-06). Commissural axon pathfinding at intermediate targets in the zebrafish forebrain. ScholarBank@NUS Repository.
Abstract: Axonal growth cones navigate using spatial and directional information derived from the extracellular environment. The responses to particular cues are not fixed; rather, intermediate targets such as the embryonic midline are only transiently attractive. This thesis explores the mechanisms of midline navigation using the zebrafish habenular commissure (HC) as a model system. This includes a study of the normal neuroanatomy and development of the HC, and techniques for labelling and manipulating zebrafish neurons. We show that the boundaries between the roof plate and the habenula are critical choice points by live imaging, localization of Eph receptors, genetic analysis and molecular manipulation. Esrom (Pam/Phr1/Highwire/RPM-1), a large intracellular signaling hub, is essential for negotiating the ipsilateral choice point, while Ryk, a Wnt co-receptor, is required at the equivalent contralateral choice point. These observations provide evidence for a two-stage model of commissure formation based on boundary navigation.
Appears in Collections:Ph.D Theses (Open)

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