Please use this identifier to cite or link to this item: https://doi.org/10.1534/genetics.106.061184
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dc.titleTrans-kingdom transposition of the maize Dissociation element
dc.contributor.authorEmelyanov, A.
dc.contributor.authorGao, Y.
dc.contributor.authorNaqvi, N.I.
dc.contributor.authorParinov, S.
dc.date.accessioned2016-11-16T11:05:51Z
dc.date.available2016-11-16T11:05:51Z
dc.date.issued2006
dc.identifier.citationEmelyanov, A., Gao, Y., Naqvi, N.I., Parinov, S. (2006). Trans-kingdom transposition of the maize Dissociation element. Genetics 174 (3) : 1095-1104. ScholarBank@NUS Repository. https://doi.org/10.1534/genetics.106.061184
dc.identifier.issn00166731
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/130427
dc.description.abstractTransposons are very valuable tools for genetic manipulation. However, the number of transposable elements that have been suitably adapted for experimental use is insufficient and the spectrum of heterologous hosts in which they have been deployed is restricted. To date, only transposons from animal hosts have been utilized in heterologous animal species and transposons of plant origin have been used in plant genetics. There has been no experimental evidence that any of the known elements could transpose in hosts belonging to both kingdoms. Here we demonstrate that the maize Dissociation (Ds) element is capable of effective Activator (Ac) transposase-mediated transposition in the zebrafish Danio rerio, yielding remarkable germline transmission rates. In addition, mammalian cells were also found to be conducive to Ds transposition. Furthermore, we demonstrate that nuclear localization of Ac transposase is essential for genomic Ds transposition. Our results support the hypothesis that Ac/Ds elements do not rely on hostspecific factors for transposition and that host factors involved in their mobility mechanism are widely conserved. Finally, even in vertebrate cells, the Ac/Ds system displays accurate transposition, largefragment carrying capacity, high transposition frequencies, efficient germline transmission, and reporter gene expression, all of which are advantageous for various genetic applications and animal biotechnology. Copyright © 2006 by the Genetics Society of America.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1534/genetics.106.061184
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1534/genetics.106.061184
dc.description.sourcetitleGenetics
dc.description.volume174
dc.description.issue3
dc.description.page1095-1104
dc.description.codenGENTA
dc.identifier.isiut000242532600004
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