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Title: CD44high memory CD8 T cells synergize with CpG DNA to activate dendritic cell IL-12p70 production
Authors: Wong, K.L.
Tang, L.F.M.
Lew, F.C.
Wong, H.S.K.
Chua, Y.L.
MacAry, P.A. 
Kemeny, D.M. 
Issue Date: 1-Jul-2009
Citation: Wong, K.L., Tang, L.F.M., Lew, F.C., Wong, H.S.K., Chua, Y.L., MacAry, P.A., Kemeny, D.M. (2009-07-01). CD44high memory CD8 T cells synergize with CpG DNA to activate dendritic cell IL-12p70 production. Journal of Immunology 183 (1) : 41-50. ScholarBank@NUS Repository.
Abstract: Protective memory CD8 T cell responses are generally associated with the rapid and efficient acquisition of CTL function. However, the ability of memory CD8 T cells to modulate immune responses through interactions with dendritic cells (DCs) during the early states of secondary Ag exposure is poorly understood. In this study, we show that murine Ag-specific CD44high CD8 T cells, representing CD8 T cells of the memory phenotype, potently activate DCs to produce high levels of IL-12p70 in conjunction with stimulation of DCs with the TLR 9 ligand, unmethylated CpG DNA. IL-12p70 production was produced predominantly by CD8α+ DCs and plasmacytoid DCs, and mediated by CD8 T cell-derived cytokines IFN-γ, GM-CSF, TNF-α, and surface CD40L. We also find that CD44high memory phenotype CD8 T cells were better DC IL-12p70 stimulators than CD44low naive phenotype CD8 T cells, and this was attributed to higher levels of IFN-γ and GM-CSF produced by CD44high memory phenotype CD8 T cells during their Ag specific interaction with DCs. Our study identifies CpG DNA as the most effective TLR ligand that cooperates with CD8 T cells for DC IL-12p70 production, and suggests that effectiveness of memory CD8 T cells could be attributed to their ability to rapidly and effectively induce protective Th1 immunity during early stages of pathogen reinfection. Copyright © 2009 by The American Association of Immunologists, Inc.
Source Title: Journal of Immunology
ISSN: 00221767
DOI: 10.4049/jimmunol.0803473
Appears in Collections:Staff Publications

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