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https://scholarbank.nus.edu.sg/handle/10635/129558
Title: | Characterization of a unique group-specific protein (U122) of the severe acute respiratory syndrome coronavirus | Authors: | Fielding, B.C. Tan, Y.-J. Shuo, S. Tan, T.H.P. Ooi, E.-E. Lim, S.G. Hong, W. Goh, P.-Y. |
Issue Date: | Jul-2004 | Citation: | Fielding, B.C., Tan, Y.-J., Shuo, S., Tan, T.H.P., Ooi, E.-E., Lim, S.G., Hong, W., Goh, P.-Y. (2004-07). Characterization of a unique group-specific protein (U122) of the severe acute respiratory syndrome coronavirus. Journal of Virology 78 (14) : 7311-7318. ScholarBank@NUS Repository. | Abstract: | A novel coronavirus (CoV) has been identified as the etiological agent of severe acute respiratory syndrome (SARS). The SARS-CoV genome encodes the characteristic essential CoV replication and structural proteins. Additionally, the genome contains six group-specific open reading frames (ORFs) larger than 50 amino acids, with no known homologues. As with the group-specific genes of the other CoVs, little is known about the SARS-CoV group-specific genes. SARS-CoV ORF7a encodes a putative unique 122-amino-acid protein, designated U122 in this study. The deduced sequence contains a probable cleaved signal sequence and a C-terminal transmembrane helix, indicating that U122 is likely to be a type I membrane protein. The C-terminal tail also contains a typical endoplasmic reticulum (ER) retrieval motif, KRKTE. U122 was expressed in SARS-CoV-infected Vero E6 cells, as it could be detected by Western blot and immunofluorescence analyses. U122 is localized to the perinuclear region of both SARS-CoV-infected and transfected cells and colocalized with ER and intermediate compartment markers. Mutational analyses showed that both the signal peptide sequence and ER retrieval motif were functional. | Source Title: | Journal of Virology | URI: | http://scholarbank.nus.edu.sg/handle/10635/129558 | ISSN: | 0022538X |
Appears in Collections: | Staff Publications |
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