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Title: A preliminary evaluation of serum proteins by capillary electrophoresis
Authors: Wong, M.-S.
Aw, T.-C. 
Keywords: Agarose gel electrophoresis
Capillary electrophoresis
Monoclonal bands
Serum proteins
Issue Date: Sep-1998
Citation: Wong, M.-S.,Aw, T.-C. (1998-09). A preliminary evaluation of serum proteins by capillary electrophoresis. Journal of Capillary Electrophoresis and Microchip Technology 5 (5-6) : 217-221. ScholarBank@NUS Repository.
Abstract: We evaluated the 5-band Serum Proteins by Capillary Electrophoresis kit (Bio-Rad Laboratories, Hercules, CA, U.S.A.) on the BioFocus 2000 CE (Bio-Rad) against conventional agarose gel electrophoresis (AGE) (Helena Laboratories, Beaumont, TX, U.S.A.). Serum from 60 patients was initially screened by AGE and divided into three groups: 1) normal electrophoretic pattern (n = 36, mean total protein 67.7 g/L), 2) monoclonal/oligoclonal gammopathy (n = 14, mean total protein 78.8 g/L), and 3) polyclonal gammopathy (n = 10, mean total protein 77.4 g/L). These samples were concurrently analyzed on the BioFocus 2000. Intraassay and interassay CVs for the five fractions for a normal sample were 0.17-1.44% and 0.42-9.11%, respectively, and 0.21-3.37% and 0.29-3.61%, respectively, for a sample with monoclonal gammopathy. Correlation coefficients for albumin and the albumin/globulin (A/G) ratio were 0.8891 (albumin range 17-93 g/L) and 0.8276 (A/G ratio range 0.39-7.81), respectively. The A/G ratio alone could not discriminate between the three groups. Capillary zone electrophoresis (CZE) correctly identified 33 of 36 samples in the normal group, and 22 of 24 samples in the other two groups, giving a clinical sensitivity and specificity of 91.7%. Our preliminary evaluation shows that protein separation by CZE is a simple, rapid, and automated alternative to conventional AGE.
Source Title: Journal of Capillary Electrophoresis and Microchip Technology
ISSN: 10795383
Appears in Collections:Staff Publications

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