Please use this identifier to cite or link to this item:
https://scholarbank.nus.edu.sg/handle/10635/129187
DC Field | Value | |
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dc.title | Effects of ouabain at low external concentrations on 86rubidium influx and specific 3H-ouabain binding in human lymphocytes in vitro | |
dc.contributor.author | Ang, L.M. | |
dc.contributor.author | Taylor, E.A. | |
dc.contributor.author | Oh, V.M.S. | |
dc.date.accessioned | 2016-11-02T03:17:49Z | |
dc.date.available | 2016-11-02T03:17:49Z | |
dc.date.issued | 1990 | |
dc.identifier.citation | Ang, L.M.,Taylor, E.A.,Oh, V.M.S. (1990). Effects of ouabain at low external concentrations on 86rubidium influx and specific 3H-ouabain binding in human lymphocytes in vitro. Asia Pacific Journal of Pharmacology 5 (1) : 51-55. ScholarBank@NUS Repository. | |
dc.identifier.issn | 02179687 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/129187 | |
dc.description.abstract | Changes in the sodium/potassium-adenosine triphosphatase (Na+/K+ pump) of human leukocytes may reflect changes in the Na+/K+ pump of vascular smooth muscle cells in cryptogenic ('essential') hypertension. Lymphocytes from healthy donors have significantly fewer Na+/K+ pumps than normal when incubated for 72 h with serum from patients with crytogenic hypertension. This effect might be due to Na+/K+ pump inhibition by a cardiac glycoside-like substance in the serum. We assessed the sensitivity of two techniques for measuring inhibition of cellular (Na+/K+ pumps by ouabain at low concentrations in vitro. We incubated lymphocytes from healthy subjects for 1 h and 72 h with unlabelled ouabain at different concentrations to determine the lowest concentration which inhibits specific 3H-ouabain binding (the number of Na+/K+ pumps) and ouabain sensitive 86rubidium influx (pump activity). Incubation of cells with ouabain for 1 h did not significantly affect Na+/K+ pump activity. The lowest ouabain concentrations which significantly reduced Na+/K+ pump numbers and activity during 72 h incubation were 5 x 10-9 and 1 x 10-8 mol L-1, which approximate the sensitivity of radioimmunoassay for plasma ouabain. We conclude that specific 3H-ouabain binding and ouabain sensitive 86rubidium influx are similarly sensitive at detecting lymphocyte Na+/K+ pump inhibition by ouabain. Both these measurements are reliable only if made when the binding of glycoside-like substances at low concentration is at equilibrium. | |
dc.source | Scopus | |
dc.type | Article | |
dc.contributor.department | MEDICINE | |
dc.description.sourcetitle | Asia Pacific Journal of Pharmacology | |
dc.description.volume | 5 | |
dc.description.issue | 1 | |
dc.description.page | 51-55 | |
dc.description.coden | APJPE | |
dc.identifier.isiut | NOT_IN_WOS | |
Appears in Collections: | Staff Publications |
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