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dc.titleEffects of ouabain at low external concentrations on 86rubidium influx and specific 3H-ouabain binding in human lymphocytes in vitro
dc.contributor.authorAng, L.M.
dc.contributor.authorTaylor, E.A.
dc.contributor.authorOh, V.M.S.
dc.identifier.citationAng, L.M.,Taylor, E.A.,Oh, V.M.S. (1990). Effects of ouabain at low external concentrations on 86rubidium influx and specific 3H-ouabain binding in human lymphocytes in vitro. Asia Pacific Journal of Pharmacology 5 (1) : 51-55. ScholarBank@NUS Repository.
dc.description.abstractChanges in the sodium/potassium-adenosine triphosphatase (Na+/K+ pump) of human leukocytes may reflect changes in the Na+/K+ pump of vascular smooth muscle cells in cryptogenic ('essential') hypertension. Lymphocytes from healthy donors have significantly fewer Na+/K+ pumps than normal when incubated for 72 h with serum from patients with crytogenic hypertension. This effect might be due to Na+/K+ pump inhibition by a cardiac glycoside-like substance in the serum. We assessed the sensitivity of two techniques for measuring inhibition of cellular (Na+/K+ pumps by ouabain at low concentrations in vitro. We incubated lymphocytes from healthy subjects for 1 h and 72 h with unlabelled ouabain at different concentrations to determine the lowest concentration which inhibits specific 3H-ouabain binding (the number of Na+/K+ pumps) and ouabain sensitive 86rubidium influx (pump activity). Incubation of cells with ouabain for 1 h did not significantly affect Na+/K+ pump activity. The lowest ouabain concentrations which significantly reduced Na+/K+ pump numbers and activity during 72 h incubation were 5 x 10-9 and 1 x 10-8 mol L-1, which approximate the sensitivity of radioimmunoassay for plasma ouabain. We conclude that specific 3H-ouabain binding and ouabain sensitive 86rubidium influx are similarly sensitive at detecting lymphocyte Na+/K+ pump inhibition by ouabain. Both these measurements are reliable only if made when the binding of glycoside-like substances at low concentration is at equilibrium.
dc.description.sourcetitleAsia Pacific Journal of Pharmacology
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