FUNCTIONAL CHARACTERIZATION OF FORMIN-DEPENDENT ACTIN POLYMERIZATION AT ADHERENS JUNCTIONS

Abstract

Cadherin-mediated cell-cell adhesion is required for epithelial tissue integrity in homeostasis, during development and in tissue repair. E-cadherin stability depends on F-actin, but the mechanisms regulating actin polymerization at cell-cell junctions remain poorly understood. Here, we investigated a role for formin-mediated actin polymerization at cell-cell junctions. We identify mDia1 and Fmnl3 as major factors enhancing actin polymerization and stabilizing E-cadherin at epithelial junctions. Fmnl3 localizes to adherens junctions downstream of Src and Cdc42, its depletion leads to a reduction in F-actin and E-cadherin at junctions, and weakening of cell-cell adhesion. Importantly, Fmnl3 expression is up-regulated and junctional localization increases during collective cell migration. Depletion of Fmnl3 or mDia1 in migrating monolayers results in dissociation of leader cells and impaired wound repair. In summary, our results show formin activity at epithelial cell-cell junctions is important for adhesion and the maintenance of epithelial cohesion during dynamic processes, such as wound repair.