Please use this identifier to cite or link to this item:
https://doi.org/10.1101/gad.211698.112
DC Field | Value | |
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dc.title | Functional small RNAs are generated from select miRNA hairpin loops in flies and mammals | |
dc.contributor.author | Okamura, K. | |
dc.contributor.author | Ladewig, E. | |
dc.contributor.author | Zhou, L. | |
dc.contributor.author | Lai, E.C. | |
dc.date.accessioned | 2016-10-22T07:45:28Z | |
dc.date.available | 2016-10-22T07:45:28Z | |
dc.date.issued | 2013-04-01 | |
dc.identifier.citation | Okamura, K., Ladewig, E., Zhou, L., Lai, E.C. (2013-04-01). Functional small RNAs are generated from select miRNA hairpin loops in flies and mammals. Genes and Development 27 (7) : 778-792. ScholarBank@NUS Repository. https://doi.org/10.1101/gad.211698.112 | |
dc.identifier.issn | 08909369 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/128887 | |
dc.description.abstract | In the canonical animal microRNA (miRNA) pathway, Drosha generates ~60- to 70-nucleotide pre-miRNA hairpins that are cleaved by Dicer into small RNA duplexes that load into Argonaute proteins, which retain a single mature strand in the active complex. The terminal loops of some miRNA hairpins regulate processing efficiency, but once liberated by Dicer, they are generally considered nonfunctional by-products. Here, we show that specific miRNA loops accumulate in effector Argonaute complexes in Drosophila and mediate miRNA-type repression. This was unexpected, since endogenous loading of Argonaute proteins was believed to occur exclusively via small RNA duplexes. Using in vitro assays, which recapitulate Argonaute-specific loop loading from synthetic pre-miRNAs and even single-stranded oligoribonucleotides corresponding to miRNA loops, we reveal that the loop-loading mechanism is distinct from duplex loading. We also show that miRNA loops loaded into the miRNA effector AGO1 are subject to 3' resection, and structure-function analyses indicate selectivity of loop loading. Finally, we demonstrate that select miRNA loops in mammals are similarly loaded into Argonaute complexes and direct target repression. Altogether, we reveal a conserved mechanism that yields functional RNAs from miRNA loop regions, broadening the repertoire of Argonaute-dependent regulatory RNAs and providing evidence for functionality of endogenous ssRNA species. © 2013 by Cold Spring Harbor Laboratory Press. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1101/gad.211698.112 | |
dc.source | Scopus | |
dc.subject | Argonaute protein | |
dc.subject | Mirna hairpin loop | |
dc.subject | Small RNA loading | |
dc.type | Article | |
dc.contributor.department | BIOLOGICAL SCIENCES | |
dc.description.doi | 10.1101/gad.211698.112 | |
dc.description.sourcetitle | Genes and Development | |
dc.description.volume | 27 | |
dc.description.issue | 7 | |
dc.description.page | 778-792 | |
dc.description.coden | GEDEE | |
dc.identifier.isiut | 000317578000007 | |
Appears in Collections: | Staff Publications |
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