Please use this identifier to cite or link to this item:
|Title:||Production of marker-free transgenic rice expressing tissue-specific Bt gene||Authors:||Qiu, C.
|Issue Date:||2010||Citation:||Qiu, C., Sangha, J.S., Song, F., Zhou, Z., Yin, A., Gu, K., Tian, D., Yang, J., Yin, Z. (2010). Production of marker-free transgenic rice expressing tissue-specific Bt gene. Plant Cell Reports 29 (10) : 1097-1107. ScholarBank@NUS Repository. https://doi.org/10.1007/s00299-010-0893-x||Abstract:||The hybrid Bacillus thuringiensis (Bt) δ-endotoxin gene Cry1Ab/Ac was used to develop a transgenic Bt rice (Oryza sativa L.) targeting lepidopteran insects of rice. Here, we show the production of a marker-free and tissue-specific expressing transgenic Bt rice line L24 using Agrobacterium-mediated transformation and a chemically regulated, Cre/loxP-mediated DNA recombination system. L24 carries a single copy of marker-free T-DNA that contains the Cry1Ab/Ac gene driven by a maize phosphoenolpyruvate carboxylase (PEPC) gene promoter. The marker-free T-DNA was integrated into the 3′ untranslated region of rice gene Os01g0154500 on the short arm of chromosome 1. Compared to the constitutive and non-specific expression of the PActin1:Cry1Ab/Ac:TNos gene in the control Bt rice line T51-1, the PPepc:Cry1Ab/Ac:TNos gene was detected only in the leaf and stem tissues of L24. More importantly, compared to high levels of CRY1Ab/Ac proteins accumulated in T51-1 seeds, the CRY1Ab/Ac proteins were not detectable in L24 seeds by Western blot analysis. As demonstrated by insect bioassay, L24 provided similar level of resistance to rice leaffolder (Cnaphalocrocis medinalis) as T51-1. The marker-free transgenic line L24 can be used directly in rice breeding for insect resistance to lepidopteran insects where absence of Bt toxin protein in the seed is highly desirable. © 2010 Springer-Verlag.||Source Title:||Plant Cell Reports||URI:||http://scholarbank.nus.edu.sg/handle/10635/128883||ISSN:||07217714||DOI:||10.1007/s00299-010-0893-x|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Sep 21, 2022
WEB OF SCIENCETM
checked on Sep 21, 2022
checked on Sep 22, 2022
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.