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Title: Production of marker-free transgenic rice expressing tissue-specific Bt gene
Authors: Qiu, C.
Sangha, J.S.
Song, F.
Zhou, Z.
Yin, A.
Gu, K.
Tian, D.
Yang, J.
Yin, Z. 
Keywords: Bt rice
Marker-free transformation
Rice leaffolder
Tissue-specific promoter
Issue Date: 2010
Citation: Qiu, C., Sangha, J.S., Song, F., Zhou, Z., Yin, A., Gu, K., Tian, D., Yang, J., Yin, Z. (2010). Production of marker-free transgenic rice expressing tissue-specific Bt gene. Plant Cell Reports 29 (10) : 1097-1107. ScholarBank@NUS Repository.
Abstract: The hybrid Bacillus thuringiensis (Bt) δ-endotoxin gene Cry1Ab/Ac was used to develop a transgenic Bt rice (Oryza sativa L.) targeting lepidopteran insects of rice. Here, we show the production of a marker-free and tissue-specific expressing transgenic Bt rice line L24 using Agrobacterium-mediated transformation and a chemically regulated, Cre/loxP-mediated DNA recombination system. L24 carries a single copy of marker-free T-DNA that contains the Cry1Ab/Ac gene driven by a maize phosphoenolpyruvate carboxylase (PEPC) gene promoter. The marker-free T-DNA was integrated into the 3′ untranslated region of rice gene Os01g0154500 on the short arm of chromosome 1. Compared to the constitutive and non-specific expression of the PActin1:Cry1Ab/Ac:TNos gene in the control Bt rice line T51-1, the PPepc:Cry1Ab/Ac:TNos gene was detected only in the leaf and stem tissues of L24. More importantly, compared to high levels of CRY1Ab/Ac proteins accumulated in T51-1 seeds, the CRY1Ab/Ac proteins were not detectable in L24 seeds by Western blot analysis. As demonstrated by insect bioassay, L24 provided similar level of resistance to rice leaffolder (Cnaphalocrocis medinalis) as T51-1. The marker-free transgenic line L24 can be used directly in rice breeding for insect resistance to lepidopteran insects where absence of Bt toxin protein in the seed is highly desirable. © 2010 Springer-Verlag.
Source Title: Plant Cell Reports
ISSN: 07217714
DOI: 10.1007/s00299-010-0893-x
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