Please use this identifier to cite or link to this item: https://doi.org/10.4161/auto.20217
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dc.titleSorting nexin Snx41 is essential for conidiation and mediates glutathione-based antioxidant defense during invasive growth in Magnaporthe oryzae
dc.contributor.authorDeng, Y.Z.
dc.contributor.authorQu, Z.
dc.contributor.authorHe, Y.
dc.contributor.authorNaqvi, N.I.
dc.date.accessioned2016-10-22T07:45:22Z
dc.date.available2016-10-22T07:45:22Z
dc.date.issued2012-07
dc.identifier.citationDeng, Y.Z., Qu, Z., He, Y., Naqvi, N.I. (2012-07). Sorting nexin Snx41 is essential for conidiation and mediates glutathione-based antioxidant defense during invasive growth in Magnaporthe oryzae. Autophagy 8 (7) : 1058-1070. ScholarBank@NUS Repository. https://doi.org/10.4161/auto.20217
dc.identifier.issn15548627
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/128878
dc.description.abstractThe sorting nexins Atg20/Snx42 and Snx41 regulate membrane traffic and endosomal protein sorting and are essential for Cvt and/or pexophagy in yeast. Previously, we showed that macroautophagy is necessary for conidiation in the rice-blast fungus Magnaporthe oryzae. Here, we analyzed the physiological function(s) of selective autophagy in Magnaporthe through targeted deletion of MGG-12832, an ortholog of yeast SNX41 and ATG20/SNX42. Loss of MGG-12832 (hereafter SNX41) abolished conidia formation and pathogenesis in M. oryzae. Snx41-GFP localized as dynamic puncta or short tubules that are partially associated with autophagosomes and/or autophagic vacuoles. PX domain, but not macroautophagy per se, was required for such localization of Snx41-GFP in Magnaporthe. Although not required for nonselective autophagy, Snx41 was essential for pexophagy in Magnaporthe. We identified Oxp1, an ATP-dependent oxoprolinase in the gamma-glutamyl cycle, as a binding partner and potential retrieval target of Snx41-dependent protein sorting. The substrate of Oxp1, 5-oxoproline, could partially restore conidiation in the snx41Δ. Exogenous glutathione, a product of the gamma-glutamyl cycle, significantly restored pathogenicity in the snx41Δ mutant, likely through counteracting the oxidative stress imposed by the host. We propose that the gamma-glutamyl cycle and glutathione biosynthesis are subject to regulation by Snx41-dependent vesicular trafficking, and mediate antioxidant defense crucial for in planta growth and pathogenic differentiation of Magnaporthe at the onset of blast disease in rice. © 2012 Landes Bioscience.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.4161/auto.20217
dc.sourceScopus
dc.subjectAntioxidant
dc.subjectAtg20
dc.subjectFungal virulence
dc.subjectGSH
dc.subjectMagnaporthe
dc.subjectSnx41
dc.subjectSorting nexin
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.4161/auto.20217
dc.description.sourcetitleAutophagy
dc.description.volume8
dc.description.issue7
dc.description.page1058-1070
dc.identifier.isiut000307830600005
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