Please use this identifier to cite or link to this item:
https://doi.org/10.4161/auto.20217
DC Field | Value | |
---|---|---|
dc.title | Sorting nexin Snx41 is essential for conidiation and mediates glutathione-based antioxidant defense during invasive growth in Magnaporthe oryzae | |
dc.contributor.author | Deng, Y.Z. | |
dc.contributor.author | Qu, Z. | |
dc.contributor.author | He, Y. | |
dc.contributor.author | Naqvi, N.I. | |
dc.date.accessioned | 2016-10-22T07:45:22Z | |
dc.date.available | 2016-10-22T07:45:22Z | |
dc.date.issued | 2012-07 | |
dc.identifier.citation | Deng, Y.Z., Qu, Z., He, Y., Naqvi, N.I. (2012-07). Sorting nexin Snx41 is essential for conidiation and mediates glutathione-based antioxidant defense during invasive growth in Magnaporthe oryzae. Autophagy 8 (7) : 1058-1070. ScholarBank@NUS Repository. https://doi.org/10.4161/auto.20217 | |
dc.identifier.issn | 15548627 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/128878 | |
dc.description.abstract | The sorting nexins Atg20/Snx42 and Snx41 regulate membrane traffic and endosomal protein sorting and are essential for Cvt and/or pexophagy in yeast. Previously, we showed that macroautophagy is necessary for conidiation in the rice-blast fungus Magnaporthe oryzae. Here, we analyzed the physiological function(s) of selective autophagy in Magnaporthe through targeted deletion of MGG-12832, an ortholog of yeast SNX41 and ATG20/SNX42. Loss of MGG-12832 (hereafter SNX41) abolished conidia formation and pathogenesis in M. oryzae. Snx41-GFP localized as dynamic puncta or short tubules that are partially associated with autophagosomes and/or autophagic vacuoles. PX domain, but not macroautophagy per se, was required for such localization of Snx41-GFP in Magnaporthe. Although not required for nonselective autophagy, Snx41 was essential for pexophagy in Magnaporthe. We identified Oxp1, an ATP-dependent oxoprolinase in the gamma-glutamyl cycle, as a binding partner and potential retrieval target of Snx41-dependent protein sorting. The substrate of Oxp1, 5-oxoproline, could partially restore conidiation in the snx41Δ. Exogenous glutathione, a product of the gamma-glutamyl cycle, significantly restored pathogenicity in the snx41Δ mutant, likely through counteracting the oxidative stress imposed by the host. We propose that the gamma-glutamyl cycle and glutathione biosynthesis are subject to regulation by Snx41-dependent vesicular trafficking, and mediate antioxidant defense crucial for in planta growth and pathogenic differentiation of Magnaporthe at the onset of blast disease in rice. © 2012 Landes Bioscience. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.4161/auto.20217 | |
dc.source | Scopus | |
dc.subject | Antioxidant | |
dc.subject | Atg20 | |
dc.subject | Fungal virulence | |
dc.subject | GSH | |
dc.subject | Magnaporthe | |
dc.subject | Snx41 | |
dc.subject | Sorting nexin | |
dc.type | Article | |
dc.contributor.department | BIOLOGICAL SCIENCES | |
dc.description.doi | 10.4161/auto.20217 | |
dc.description.sourcetitle | Autophagy | |
dc.description.volume | 8 | |
dc.description.issue | 7 | |
dc.description.page | 1058-1070 | |
dc.identifier.isiut | 000307830600005 | |
Appears in Collections: | Staff Publications |
Show simple item record
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.