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|Title:||Quorum sensing signal interference within and across the kingdoms||Authors:||YANG FAN||Keywords:||quorum sensing; signal interference; acyl-homoserine lactone; AHL-lactonase; Pseudomonas aeruginosa; paraoxonase||Issue Date:||3-Dec-2008||Citation:||YANG FAN (2008-12-03). Quorum sensing signal interference within and across the kingdoms. ScholarBank@NUS Repository.||Abstract:||Quorum sensing (QS) is known to be widespread in prokaryotic world for regulating numerous important biological activities. Consequently, anti-QS approaches have recently been proposed as a promising strategy for development of novel antimicrobial agents by uncoupling bacterial QS. In this study, I explored further on the QS signal interference within and across the kingdoms. The microbial diversity in multi-species biofilms was inspected and both QS signal producing and degrading bacterial species were identified. A putative QS inhibitor (PAi), isolated from Pseudomonas aeruginosa has been purified and characterized as 2-amino-4-methoxy-but-3-enoic acid. The molecular mechanisms of PAi production were also investigated. A novel enzymic anti-QS mechanism was identified from eukaryotic kingdom. Further analysis revealed that mammalian sera possess a widely conserved catalytic mechanism different from bacterial AHL-lactonase, although they share a similar function in degrading QS signals.||URI:||http://scholarbank.nus.edu.sg/handle/10635/12886|
|Appears in Collections:||Ph.D Theses (Open)|
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