Please use this identifier to cite or link to this item: https://doi.org/10.1007/s12017-012-8170-5
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dc.titleAxon myelination and electrical stimulation in a microfluidic, compartmentalized cell culture platform
dc.contributor.authorYang, I.H.
dc.contributor.authorGary, D.
dc.contributor.authorMalone, M.
dc.contributor.authorDria, S.
dc.contributor.authorHoudayer, T.
dc.contributor.authorBelegu, V.
dc.contributor.authorMcDonald, J.W.
dc.contributor.authorThakor, N.
dc.date.accessioned2016-10-19T08:44:56Z
dc.date.available2016-10-19T08:44:56Z
dc.date.issued2012-06
dc.identifier.citationYang, I.H., Gary, D., Malone, M., Dria, S., Houdayer, T., Belegu, V., McDonald, J.W., Thakor, N. (2012-06). Axon myelination and electrical stimulation in a microfluidic, compartmentalized cell culture platform. NeuroMolecular Medicine 14 (2) : 112-118. ScholarBank@NUS Repository. https://doi.org/10.1007/s12017-012-8170-5
dc.identifier.issn15351084
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/128767
dc.description.abstractAxon demyelination contributes to the loss of sensory and motor function following injury or disease in the central nervous system. Numerous reports have demonstrated that myelination can be achieved in neuron/oligodendrocyte co-cultures.However, the ability to selectively treat neuron or oligodendrocyte (OL) cell bodies in co-cultures improves the value of these systems when designing mechanism-based therapeutics. We have developed a microfluidic-based compartmentalized culture system to achieve segregation of neuron and OL cell bodies while simultaneously allowing the formation ofmyelin sheaths. Ourmicrofluidic platformallows for a high replicate number, minimal leakage, and high flexibility. Using a custom built lid, fit with platinum electrodes for electrical stimulation (10-Hz pulses at a constant 3 V with ∼190 kΩ impedance), we employed the microfluidic platform to achieve activity-dependent myelin segment formation. Electrical stimulation of dorsal root ganglia resulted in a fivefold increase in the number of myelinated segments/mm 2 when compared to unstimulated controls (19.6 ± 3.0 vs. 3.6 ± 2.3 MBP + segments/mm 2). This work describes the modification of a microfluidic, multi-chamber system so that electrical stimulation can be used to achieve increased levels of myelination while maintaining control of the cell culture microenvironment. © Springer Science+Business Media, LLC 2012.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1007/s12017-012-8170-5
dc.sourceScopus
dc.subjectElectrical stimulation
dc.subjectMicrofluidic device
dc.subjectMyelination
dc.subjectOligodendrocyte
dc.typeArticle
dc.contributor.departmentLIFE SCIENCES INSTITUTE
dc.contributor.departmentELECTRICAL ENGINEERING
dc.description.doi10.1007/s12017-012-8170-5
dc.description.sourcetitleNeuroMolecular Medicine
dc.description.volume14
dc.description.issue2
dc.description.page112-118
dc.description.codenNMEEA
dc.identifier.isiut000303871200002
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