Please use this identifier to cite or link to this item: https://doi.org/10.1038/nprot.2011.304
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dc.titleMultisubstrate-compatible ELISA procedures for rapid and high-sensitivity immunoassays
dc.contributor.authorDixit, C.K.
dc.contributor.authorVashist, S.K.
dc.contributor.authorMacCraith, B.D.
dc.contributor.authorO'Kennedy, R.
dc.date.accessioned2016-10-18T06:27:39Z
dc.date.available2016-10-18T06:27:39Z
dc.date.issued2011-03
dc.identifier.citationDixit, C.K., Vashist, S.K., MacCraith, B.D., O'Kennedy, R. (2011-03). Multisubstrate-compatible ELISA procedures for rapid and high-sensitivity immunoassays. Nature Protocols 6 (4) : 439-445. ScholarBank@NUS Repository. https://doi.org/10.1038/nprot.2011.304
dc.identifier.issn17542189
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/128551
dc.description.abstractThis protocol describes an improved and optimized approach to develop rapid and high-sensitivity ELISAs by covalently immobilizing antibody on chemically modified polymeric surfaces. The method involves initial surface activation with KOH and an O 2 plasma, and then amine functionalization with 3-aminopropyltriethoxysilane. The second step requires covalent antibody immobilization on the aminated surface, followed by ELISA. The ELISA procedure developed is 16-fold more sensitive than established methods. This protocol could be used generally as a quantitative analytical approach to perform high-sensitivity and rapid assays in clinical situations, and would provide a faster approach to screen phage-displayed libraries in antibody development facilities. The antibody immobilization procedure is of ∼3 h duration and facilitates rapid ELISAs. This method can be used to perform assays on a wide range of commercially relevant solid support matrices (including those that are chemically inert) with various biosensor formats. © 2011 Nature America, Inc. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1038/nprot.2011.304
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentNUS NANOSCIENCE & NANOTECH INITIATIVE
dc.description.doi10.1038/nprot.2011.304
dc.description.sourcetitleNature Protocols
dc.description.volume6
dc.description.issue4
dc.description.page439-445
dc.identifier.isiut000288993200003
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