Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.bpj.2012.09.004
Title: Characterization of curli a production on living bacterial surfaces by scanning probe microscopy
Authors: Oh, Y.J.
Cui, Y.
Kim, H.
Li, Y.
Hinterdorfer, P.
Park, S. 
Issue Date: 17-Oct-2012
Citation: Oh, Y.J., Cui, Y., Kim, H., Li, Y., Hinterdorfer, P., Park, S. (2012-10-17). Characterization of curli a production on living bacterial surfaces by scanning probe microscopy. Biophysical Journal 103 (8) : 1666-1671. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bpj.2012.09.004
Abstract: Curli are adhesive surface fibers produced by many Enterobacteriaceae, such as Escherichia coli and Salmonella enterica. They are implicated in bacterial attachment and invasion to epithelial cells. In this study, atomic force microscopy was used to determine the effects of curli on topology and mechanical properties of live E. coli cells. Young's moduli of both curli-deficient and curli-overproducing mutants were significantly lower than that of their wild-type (WT) strain, while decay lengths of the former strains were higher than that of the latter strain. Surprisingly, topological images showed that, unlike the WT and curli-overproducing mutant, the curli-deficient mutant produced a large number of flagella-like fibers, which may explain why the strain had a lower Young's modulus than the WT. These results suggest that the mechanical properties of bacterial surfaces are greatly affected by the presence of filamentous structures such as curli and flagella. © 2012 Biophysical Society.
Source Title: Biophysical Journal
URI: http://scholarbank.nus.edu.sg/handle/10635/128516
ISSN: 00063495
DOI: 10.1016/j.bpj.2012.09.004
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

13
checked on Aug 20, 2019

WEB OF SCIENCETM
Citations

9
checked on Aug 12, 2019

Page view(s)

17
checked on Aug 16, 2019

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.