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Title: Analysis of changes in gene expression and metabolic profiles induced by silica-coated magnetic nanoparticles
Authors: Shim, W.
Paik, M.J.
Nguyen, D.-T.
Lee, J.-K.
Lee, Y.
Kim, J.-H.
Shin, E.-H.
Kang, J.S.
Jung, H.-S.
Choi, S.
Park, S. 
Shim, J.S.
Lee, G.
Keywords: cytotoxicity
magnetic nanoparticles
Issue Date: 25-Sep-2012
Citation: Shim, W., Paik, M.J., Nguyen, D.-T., Lee, J.-K., Lee, Y., Kim, J.-H., Shin, E.-H., Kang, J.S., Jung, H.-S., Choi, S., Park, S., Shim, J.S., Lee, G. (2012-09-25). Analysis of changes in gene expression and metabolic profiles induced by silica-coated magnetic nanoparticles. ACS Nano 6 (9) : 7665-7680. ScholarBank@NUS Repository.
Abstract: Magnetic nanoparticles (MNPs) have proven themselves to be useful in biomedical research; however, previous reports were insufficient to address the potential dangers of nanoparticles. Here, we investigated gene expression and metabolic changes based on the microarray and gas chromatography-mass spectrometry with human embryo kidney 293 cells treated with MNPs@SiO 2(RITC), a silica-coated MNP containing Rhodamine B isothiocyanate (RITC). In addition, measurement of reactive oxygen species (ROS) and ATP analysis were performed to evaluate the effect of MNPs@SiO 2(RITC) on mitochondrial function. Compared to the nontreated control, glutamic acid was increased by more than 2.0-fold, and expression of genes related to the glutamic acid metabolic pathway was also disturbed in 1.0 μg/μL of MNPs@SiO 2(RITC)-treated cells. Furthermore, increases in ROS concentration and mitochondrial damage were observed in this MNPs@SiO 2(RITC) concentration. The organic acids related to the Krebs cycle were also disturbed, and the capacity of ATP synthesis was decreased in cell treated with an overdose of MNPs@SiO 2(RITC). Collectively, these results suggest that overdose (1.0 μg/μL) of MNPs caused transcriptomic and metabolic disturbance. In addition, we suggest that a combination of gene expression and metabolic profiles will provide more detailed and sensitive toxicological evaluation for nanoparticles. © 2012 American Chemical Society.
Source Title: ACS Nano
ISSN: 19360851
DOI: 10.1021/nn301113f
Appears in Collections:Staff Publications

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