Please use this identifier to cite or link to this item: https://doi.org/10.1104/pp.114.236430
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dc.titleMultigene engineering of triacylglycerol metabolism boosts seed oil content in Arabidopsis
dc.contributor.authorvan Erp, H.
dc.contributor.authorKelly, A.A.
dc.contributor.authorMenard, G.
dc.contributor.authorEastmond, P.J.
dc.date.accessioned2016-10-18T06:27:06Z
dc.date.available2016-10-18T06:27:06Z
dc.date.issued2014
dc.identifier.citationvan Erp, H., Kelly, A.A., Menard, G., Eastmond, P.J. (2014). Multigene engineering of triacylglycerol metabolism boosts seed oil content in Arabidopsis. Plant Physiology 165 (1) : 30-36. ScholarBank@NUS Repository. https://doi.org/10.1104/pp.114.236430
dc.identifier.issn15322548
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/128506
dc.description.abstractIncreasing the yield of oilseed crops is an important objective for biotechnologists. A number of individual genes involved in triacylglycerol metabolism have previously been reported to enhance the oil content of seeds when their expression is altered. However, it has yet to be established whether specific combinations of these genes can be used to achieve an additive effect and whether this leads to enhanced yield. Using Arabidopsis (Arabidopsis thaliana) as an experimental system, we show that seed-specific overexpression of WRINKLED1 (a transcriptional regulator of glycolysis and fatty acid synthesis) and DIACYLGLYCEROL ACYLTRANSFERASE1 (a triacylglycerol biosynthetic enzyme) combined with suppression of the triacylglycerol lipase SUGAR-DEPENDENT1 results in a higher percentage seed oil content and greater seed mass than manipulation of each gene individually. Analysis of total seed yield per plant suggests that, despite a reduction in seed number, the total yield of oil is also increased. © 2014 American Society of Plant Biologists. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1104/pp.114.236430
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentLIFE SCIENCES INSTITUTE
dc.description.doi10.1104/pp.114.236430
dc.description.sourcetitlePlant Physiology
dc.description.volume165
dc.description.issue1
dc.description.page30-36
dc.description.codenPLPHA
dc.identifier.isiut000335906300003
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