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https://scholarbank.nus.edu.sg/handle/10635/128412
DC Field | Value | |
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dc.title | ANALYSIS OF TRANSCIPTOME AND SPLICING CHANGES IN ZEBRAFISH MODEL FOR SPINAL MUSCULAR ATROPHY | |
dc.contributor.author | HIMANSHU VYAS | |
dc.date.accessioned | 2016-10-13T18:00:20Z | |
dc.date.available | 2016-10-13T18:00:20Z | |
dc.date.issued | 2016-01-21 | |
dc.identifier.citation | HIMANSHU VYAS (2016-01-21). ANALYSIS OF TRANSCIPTOME AND SPLICING CHANGES IN ZEBRAFISH MODEL FOR SPINAL MUSCULAR ATROPHY. ScholarBank@NUS Repository. | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/128412 | |
dc.description.abstract | Spinal Muscular Atrophy (SMA) is a human neurodegenerative disease that leads to motor neuron loss and paralysis. SMA is caused by reduced levels of the ubiquitously expressed protein, Survival Motor Neuron (SMN). It remains unclear how a SMN deficiency leads to an apparently motor neuron specific phenotype and whether other cell types are also involved in SMA pathology. In this study, I applied RNA sequencing to analyse the transcriptomes of motor neurons and Schwann cells in a zebrafish SMA model. I identified transcripts that are alternatively spliced in both cell types and are implicated in the maintenance of neuronal function. CRISPR/Cas was used to functionally characterize the most promising candidate identified by RNAseq. Taken together, I propose that SMN deficiency causes transcriptomic changes and altered splicing in motor neurons and Schwann cells that affects their physiology, and that Schwann cells might contribute to SMA pathology in a non-cell autonomous manner. | |
dc.language.iso | en | |
dc.subject | Zebrafish, Spinal muscular atrophy, RNAseq, FACS, motor neuron, Schwann cells | |
dc.type | Thesis | |
dc.contributor.department | BIOLOGICAL SCIENCES | |
dc.contributor.supervisor | WINKLER, CHRISTOPH WOLFRAM | |
dc.description.degree | Ph.D | |
dc.description.degreeconferred | DOCTOR OF PHILOSOPHY | |
dc.identifier.isiut | NOT_IN_WOS | |
Appears in Collections: | Ph.D Theses (Open) |
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VyasH.pdf | 4.02 MB | Adobe PDF | OPEN | None | View/Download |
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