Please use this identifier to cite or link to this item: https://doi.org/10.1002/pd.2946
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dc.titleSame-day prenatal diagnosis of common chromosomal aneuploidies using microfluidics-fluorescence in situ hybridization
dc.contributor.authorHo, S.S.Y.
dc.contributor.authorChua, C.
dc.contributor.authorGole, L.
dc.contributor.authorBiswas, A.
dc.contributor.authorKoay, E.
dc.contributor.authorChoolani, M.
dc.date.accessioned2016-09-07T03:10:13Z
dc.date.available2016-09-07T03:10:13Z
dc.date.issued2012-04
dc.identifier.citationHo, S.S.Y., Chua, C., Gole, L., Biswas, A., Koay, E., Choolani, M. (2012-04). Same-day prenatal diagnosis of common chromosomal aneuploidies using microfluidics-fluorescence in situ hybridization. Prenatal Diagnosis 32 (4) : 321-328. ScholarBank@NUS Repository. https://doi.org/10.1002/pd.2946
dc.identifier.issn01973851
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/127012
dc.description.abstractRapid molecular prenatal diagnostic methods, such as fluorescence in situ hybridization (FISH), quantitative fluorescence-PCR, and multiplex ligation-dependent probe amplification, can detect common fetal aneuploidies within 24 to 48h. However, specific diagnosis or aneuploidy exclusion should be ideally available within the same day as fetal sampling to alleviate parental anxiety. Microfluidic technologies integrate different steps into a microchip, saving time and costs. We have developed a cost-effective, same-day prenatal diagnostic FISH assay using microfluidics. Amniotic fluids (1-4mL from 40 pregnant women at 15-22 weeks of gestation) were fixed with Carnoy's before loading into the microchannels of a microfluidic FISH-integrated nanostructured device. The glass slides were coated with nanostructured titanium dioxide to facilitate cell adhesion. Pretreatment and hybridization were performed within the microchannels. Fifty nuclei were counted by two independent analysts, and all results were validated with their respective karyotypes. Of the 40 samples, we found three cases of fetal aneuploidies (trisomies 13, 18, and 21), whereas the remaining 37 cases were normal. Results were concordant with their karyotypes and ready to be released within 3h of sample receipt. Microfluidic FISH, using 20-fold less than the recommended amount of probe, is a cost-effective method to diagnose common fetal aneuploidies within the same day of fetal sampling. © 2011 John Wiley & Sons, Ltd.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1002/pd.2946
dc.sourceScopus
dc.subjectFetal cells
dc.subjectFetal ultrasound
dc.subjectFish
dc.subjectGeneral cytogenetics
dc.subjectGenetic counselling
dc.subjectMLPA
dc.typeArticle
dc.contributor.departmentOBSTETRICS & GYNAECOLOGY
dc.contributor.departmentPATHOLOGY
dc.description.doi10.1002/pd.2946
dc.description.sourcetitlePrenatal Diagnosis
dc.description.volume32
dc.description.issue4
dc.description.page321-328
dc.description.codenPRDID
dc.identifier.isiut000302149400004
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