Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.canlet.2011.03.001
Title: Synergistic effect of low-dose cucurbitacin B and low-dose methotrexate for treatment of human osteosarcoma
Authors: Lee, D.H.
Thoennissen, N.H.
Goff, C.
Iwanski, G.B.
Forscher, C.
Doan, N.B.
Said, J.W.
Phillip Koeffler, H. 
Keywords: Cucurbitacin b
Methotrexate
Osteosarcoma
Synergism
Xenograft
Issue Date: 28-Jul-2011
Citation: Lee, D.H., Thoennissen, N.H., Goff, C., Iwanski, G.B., Forscher, C., Doan, N.B., Said, J.W., Phillip Koeffler, H. (2011-07-28). Synergistic effect of low-dose cucurbitacin B and low-dose methotrexate for treatment of human osteosarcoma. Cancer Letters 306 (2) : 161-170. ScholarBank@NUS Repository. https://doi.org/10.1016/j.canlet.2011.03.001
Abstract: We investigated the use of cucurbitacin B, a plant-derived tetracyclic triterpenoid, as a single agent or in combination with methotrexate (MTX) for human osteosarcoma (OS) treatment. Cucurbitacin B showed antiproliferative activity against seven human OS cell lines in vitro accompanying G2/M cell cycle arrest, apoptosis, and inhibition of ERK, Akt, and mTOR proteins. Cucurbitacin B in combination with MTX synergistically inhibited OS cell growth in vitro. Low-dose cucurbitacin B (LD-CuB, 0.5. mg/kg body weight) or low-dose MTX (LD-MTX, 150. mg/kg) failed to decrease the size of human OS xenografts in nude mice. However, combined therapy at identical concentrations inhibited tumor growth by 62% vs. LD-CuB and 81% vs. LD-MTX (p< 0.001). Strikingly, the effect persisted even when the dose of MTX was decreased by two thirds (VLD-MTX, 50. mg/kg). In conclusion, cucurbitacin B alone or in combination with MTX shows promising antiproliferative activity against human OS. © 2011 Elsevier Ireland Ltd.
Source Title: Cancer Letters
URI: http://scholarbank.nus.edu.sg/handle/10635/126862
ISSN: 03043835
DOI: 10.1016/j.canlet.2011.03.001
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