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https://doi.org/10.1016/j.canlet.2011.03.001
Title: | Synergistic effect of low-dose cucurbitacin B and low-dose methotrexate for treatment of human osteosarcoma | Authors: | Lee, D.H. Thoennissen, N.H. Goff, C. Iwanski, G.B. Forscher, C. Doan, N.B. Said, J.W. Phillip Koeffler, H. |
Keywords: | Cucurbitacin b Methotrexate Osteosarcoma Synergism Xenograft |
Issue Date: | 28-Jul-2011 | Citation: | Lee, D.H., Thoennissen, N.H., Goff, C., Iwanski, G.B., Forscher, C., Doan, N.B., Said, J.W., Phillip Koeffler, H. (2011-07-28). Synergistic effect of low-dose cucurbitacin B and low-dose methotrexate for treatment of human osteosarcoma. Cancer Letters 306 (2) : 161-170. ScholarBank@NUS Repository. https://doi.org/10.1016/j.canlet.2011.03.001 | Abstract: | We investigated the use of cucurbitacin B, a plant-derived tetracyclic triterpenoid, as a single agent or in combination with methotrexate (MTX) for human osteosarcoma (OS) treatment. Cucurbitacin B showed antiproliferative activity against seven human OS cell lines in vitro accompanying G2/M cell cycle arrest, apoptosis, and inhibition of ERK, Akt, and mTOR proteins. Cucurbitacin B in combination with MTX synergistically inhibited OS cell growth in vitro. Low-dose cucurbitacin B (LD-CuB, 0.5. mg/kg body weight) or low-dose MTX (LD-MTX, 150. mg/kg) failed to decrease the size of human OS xenografts in nude mice. However, combined therapy at identical concentrations inhibited tumor growth by 62% vs. LD-CuB and 81% vs. LD-MTX (p< 0.001). Strikingly, the effect persisted even when the dose of MTX was decreased by two thirds (VLD-MTX, 50. mg/kg). In conclusion, cucurbitacin B alone or in combination with MTX shows promising antiproliferative activity against human OS. © 2011 Elsevier Ireland Ltd. | Source Title: | Cancer Letters | URI: | http://scholarbank.nus.edu.sg/handle/10635/126862 | ISSN: | 03043835 | DOI: | 10.1016/j.canlet.2011.03.001 |
Appears in Collections: | Staff Publications |
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