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Title: Relationships between cholesterol efflux and high-density lipoprotein particles in patients with type 2 diabetes mellitus
Authors: Tan, H.C.
Tai, E.S. 
Sviridov, D.
Nestel, P.J.
Ng, C.
Chan, E.
Teo, Y.
Wai, D.C.H.
Keywords: ApoB-depleted plasma
Diabetic dyslipidemia
HDL particles
Nuclear magnetic resonance
Reverse cholesterol transport
Issue Date: Nov-2011
Citation: Tan, H.C., Tai, E.S., Sviridov, D., Nestel, P.J., Ng, C., Chan, E., Teo, Y., Wai, D.C.H. (2011-11). Relationships between cholesterol efflux and high-density lipoprotein particles in patients with type 2 diabetes mellitus. Journal of Clinical Lipidology 5 (6) : 467-473. ScholarBank@NUS Repository.
Abstract: Background: High-density lipoprotein (HDL) encompasses a heterogeneous population of lipoproteins with differences in functionality. The impact of HDL heterogeneity on its ability to support HDL-mediated cholesterol efflux has not been previously studied in patients with type 2 diabetes mellitus (T2DM). Objectives: To examine the relationships between various HDL subtypes and cholesterol efflux from macrophages in patients with T2DM. Methods: Lipoprotein molecular profiles of 44 patients were studied by NMR spectroscopy. Cholesterol efflux was expressed as percentage efflux of radioactivity from lipid-laden THP-1 macrophages preincubated with 3H-cholesterol and then incubated with serum depleted of apolipoprotein B to provide an HDL-enriched acceptor medium. Results: There was a predominance of small HDL particles (59%) and small putatively atherogenic low-density lipoprotein particles (56%). Neither HDL-C nor ApoA-I concentrations showed statistically significant correlations with percentage cholesterol efflux, but a significant positive relationship was found with the total HDL particle concentration (r = 0.41, P =.005) contributed to largely by medium HDL particles (r = 0.41, P =.006). The correlation between medium-sized HDL particle concentration remained significantly associated with cholesterol efflux when assessed with the use of a linear regression model that included all the HDL lipoprotein subclass concentrations as well as apolipoprotein A-I. Importantly, no statistically significant association was observed between the number of small HDL particles and cholesterol efflux. Hemoglobin A1c showed a significant inverse correlation with cholesterol efflux (r = -0.31, P =.04). Conclusion: In patients with moderately controlled type 2 diabetes mellitus, cholesterol efflux from macrophages incubated with apolipoprotein B-depleted plasmas correlated significantly and positively with the concentration of total and medium-sized HDL and not with that of the smallest particles. © 2011 National Lipid Association. All rights reserved.
Source Title: Journal of Clinical Lipidology
ISSN: 19332874
DOI: 10.1016/j.jacl.2011.06.016
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