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Title: Increased viral quasispecies evolution in HBeAg seroconverter patients treated with oral nucleoside therapy
Authors: Cheng, Y. 
Guindon, S.
Rodrigo, A.
Lim, S.G. 
Keywords: HBV DNA
Nucleoside analogues
Phylogenetic tree
Positive selection
Precore/core mutations
Viral quasispecies
Viral variants
Issue Date: Feb-2013
Citation: Cheng, Y., Guindon, S., Rodrigo, A., Lim, S.G. (2013-02). Increased viral quasispecies evolution in HBeAg seroconverter patients treated with oral nucleoside therapy. Journal of Hepatology 58 (2) : 217-224. ScholarBank@NUS Repository.
Abstract: Background & Aims: Increased viral diversity and evolution appear to be a pre-HBeAg-seroconversion feature in spontaneous and interferon-treated seroconverters. The aim of this study was to examine the viral evolution pattern in nucleoside analogue related HBeAg-seroconversion. Methods: This was a case control study consisting of ten lamivudine-treated HBeAg-seroconverters and ten lamivudine-treated non-seroconverters as matching controls. All patients in this study were followed as long as 6 years after starting lamivudine, and cases had three serum time points before HBeAg-seroconversion while controls had three matching serum time points. Nested PCR, cloning and sequencing of HBV precore/core gene were performed. Sequences were aligned with Clustal X 2.0. Phylogenetic trees were constructed and viral diversity, evolutionary rates and patterns of positive selection were evaluated. Results: After starting lamivudine treatment, HBV viral diversity increased in both seroconverters and non-seroconverters, but seroconverters showed a significantly higher level of viral diversity that persisted over time by 2.1-fold (p = 0.009). The increased viral diversity correlated with reduced HBV DNA levels (p
Source Title: Journal of Hepatology
ISSN: 01688278
DOI: 10.1016/j.jhep.2012.09.017
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