Please use this identifier to cite or link to this item:
|Title:||Rapid purification of recombinant dengue and West Nile virus envelope Domain III proteins by metal affinity membrane chromatography||Authors:||Tan, L.C.M.
Envelope Domain III
West Nile virus
|Issue Date:||Nov-2010||Citation:||Tan, L.C.M., Chua, A.J.S., Goh, L.S.L., Pua, S.M., Cheong, Y.K., Ng, M.L. (2010-11). Rapid purification of recombinant dengue and West Nile virus envelope Domain III proteins by metal affinity membrane chromatography. Protein Expression and Purification 74 (1) : 129-137. ScholarBank@NUS Repository. https://doi.org/10.1016/j.pep.2010.06.015||Abstract:||Arthropod-borne flaviviruses such as dengue virus (DENV) and West Nile virus (WNV) pose significant health threats to the global community. Due to escalating numbers of DENV and WNV infections worldwide, development of an effective vaccine remains a global health priority. As flavivirus envelope Domain III (DIII) protein is highly immunogenic and capable of inducing neutralizing antibodies against wild-type virus, it is both a potential protein subunit vaccine candidate and a suitable diagnostic reagent. Here, we describe the use of metal affinity membrane chromatography as a rapid and improved alternative for the purification of recombinant DIII (rDIII) antigens from DENV serotypes 1-4 and WNV - New York, Sarafend, Wengler and Kunjin strains. Optimum conditions for the expression, solubilization, renaturation and purification of these proteins were established. The purified proteins were confirmed by MALDI-TOF mass spectrometry and ELISA using antibodies raised against the respective viruses. Biological function of the purified rDIII proteins was confirmed by their ability to generate DIII-specific antibodies in mice that could neutralize the virus. © 2010 Elsevier Inc. All rights reserved.||Source Title:||Protein Expression and Purification||URI:||http://scholarbank.nus.edu.sg/handle/10635/126748||ISSN:||10465928||DOI:||10.1016/j.pep.2010.06.015|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.