Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.micinf.2013.10.002
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dc.titleProtective role of adenylate cyclase in the context of a live pertussis vaccine candidate
dc.contributor.authorLim, A.
dc.contributor.authorNg, J.K.W.
dc.contributor.authorLocht, C.
dc.contributor.authorAlonso, S.
dc.date.accessioned2016-09-06T08:19:22Z
dc.date.available2016-09-06T08:19:22Z
dc.date.issued2014-01
dc.identifier.citationLim, A., Ng, J.K.W., Locht, C., Alonso, S. (2014-01). Protective role of adenylate cyclase in the context of a live pertussis vaccine candidate. Microbes and Infection 16 (1) : 51-60. ScholarBank@NUS Repository. https://doi.org/10.1016/j.micinf.2013.10.002
dc.identifier.issn12864579
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/126747
dc.description.abstractDespite high vaccination coverage, pertussis remains an important respiratory infectious disease and the least-controlled vaccine-preventable infectious disease in children. Natural infection with Bordetella pertussis is known to induce strong and long-lasting immunity that wanes later than vaccine-mediated immunity. Therefore, a live attenuated B. pertussis vaccine, named BPZE1, has been developed and has recently completed a phase I clinical trial in adult human volunteers. In this study, we investigated the contribution of adenylate cyclase (CyaA) in BPZE1-mediated protection against pertussis. A CyaA-deficient BPZE1 mutant was thus constructed. Absence of CyaA did not compromise the adherence properties of the bacteria onto mammalian cells. However, the CyaA-deficient mutant displayed a slight impairment in the ability to survive within macrophages compared to the parental BPZE1 strain. Invivo, whereas the protective efficacy of the CyaA-deficient mutant was comparable to the parental strain at a vaccine dose of 5×105colony forming units (CFU), it was significantly impaired at a vaccine dose of 5×103CFU. This impairment correlated with impaired lung colonization ability, and impaired IFN-γ production in the animal immunized with the CyaA-deficient BPZE1 mutant while the pertussis-specific antibody profile and Th17 response were comparable to those observed in BPZE1-immunized mice. Our findings thus support a role of CyaA in BPZE1-mediated protection through induction of cellular mediated immunity. © 2013 Institut Pasteur.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.micinf.2013.10.002
dc.sourceScopus
dc.subjectAdenylate cyclase
dc.subjectBordetella pertussis
dc.subjectBPZE1
dc.typeArticle
dc.contributor.departmentMICROBIOLOGY
dc.description.doi10.1016/j.micinf.2013.10.002
dc.description.sourcetitleMicrobes and Infection
dc.description.volume16
dc.description.issue1
dc.description.page51-60
dc.description.codenMCINF
dc.identifier.isiut000347864500006
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