Please use this identifier to cite or link to this item:
https://doi.org/10.1016/j.micinf.2013.10.002
DC Field | Value | |
---|---|---|
dc.title | Protective role of adenylate cyclase in the context of a live pertussis vaccine candidate | |
dc.contributor.author | Lim, A. | |
dc.contributor.author | Ng, J.K.W. | |
dc.contributor.author | Locht, C. | |
dc.contributor.author | Alonso, S. | |
dc.date.accessioned | 2016-09-06T08:19:22Z | |
dc.date.available | 2016-09-06T08:19:22Z | |
dc.date.issued | 2014-01 | |
dc.identifier.citation | Lim, A., Ng, J.K.W., Locht, C., Alonso, S. (2014-01). Protective role of adenylate cyclase in the context of a live pertussis vaccine candidate. Microbes and Infection 16 (1) : 51-60. ScholarBank@NUS Repository. https://doi.org/10.1016/j.micinf.2013.10.002 | |
dc.identifier.issn | 12864579 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/126747 | |
dc.description.abstract | Despite high vaccination coverage, pertussis remains an important respiratory infectious disease and the least-controlled vaccine-preventable infectious disease in children. Natural infection with Bordetella pertussis is known to induce strong and long-lasting immunity that wanes later than vaccine-mediated immunity. Therefore, a live attenuated B. pertussis vaccine, named BPZE1, has been developed and has recently completed a phase I clinical trial in adult human volunteers. In this study, we investigated the contribution of adenylate cyclase (CyaA) in BPZE1-mediated protection against pertussis. A CyaA-deficient BPZE1 mutant was thus constructed. Absence of CyaA did not compromise the adherence properties of the bacteria onto mammalian cells. However, the CyaA-deficient mutant displayed a slight impairment in the ability to survive within macrophages compared to the parental BPZE1 strain. Invivo, whereas the protective efficacy of the CyaA-deficient mutant was comparable to the parental strain at a vaccine dose of 5×105colony forming units (CFU), it was significantly impaired at a vaccine dose of 5×103CFU. This impairment correlated with impaired lung colonization ability, and impaired IFN-γ production in the animal immunized with the CyaA-deficient BPZE1 mutant while the pertussis-specific antibody profile and Th17 response were comparable to those observed in BPZE1-immunized mice. Our findings thus support a role of CyaA in BPZE1-mediated protection through induction of cellular mediated immunity. © 2013 Institut Pasteur. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.micinf.2013.10.002 | |
dc.source | Scopus | |
dc.subject | Adenylate cyclase | |
dc.subject | Bordetella pertussis | |
dc.subject | BPZE1 | |
dc.type | Article | |
dc.contributor.department | MICROBIOLOGY | |
dc.description.doi | 10.1016/j.micinf.2013.10.002 | |
dc.description.sourcetitle | Microbes and Infection | |
dc.description.volume | 16 | |
dc.description.issue | 1 | |
dc.description.page | 51-60 | |
dc.description.coden | MCINF | |
dc.identifier.isiut | 000347864500006 | |
Appears in Collections: | Staff Publications |
Show simple item record
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.