Please use this identifier to cite or link to this item:
|Title:||Interferon-induced SCYL2 limits release of HIV-1 by triggering PP2A-mediated dephosphorylation of the viral protein Vpu||Authors:||Miyakawa, K.
|Issue Date:||9-Oct-2012||Citation:||Miyakawa, K., Sawasaki, T., Matsunaga, S., Tokarev, A., Quinn, G., Kimura, H., Nomaguchi, M., Adachi, A., Yamamoto, N., Guatelli, J., Ryo, A. (2012-10-09). Interferon-induced SCYL2 limits release of HIV-1 by triggering PP2A-mediated dephosphorylation of the viral protein Vpu. Science Signaling 5 (245) : -. ScholarBank@NUS Repository. https://doi.org/10.1126/scisignal.2003212||Abstract:||Human cells respond to infection by retroviruses through the actions of proteins that inhibit the spread of viruses to other cells. One example is bone marrow stromal cell antigen 2 (BST2; also known as tetherin), which is an interferon (IFN)-inducible protein that restricts the release of progeny virions from infected cells. The HIV-1 accessory protein Vpu (viral protein U) causes degradation of BST2, and phosphorylation of Vpu at residues Ser52 and Ser56 is required for this function. We report that the host protein SCY1-like protein 2 (SCYL2) mediates the dephosphorylation of Vpu, antagonizing Vpu function and facilitating BST2-dependent restriction of HIV-1 release. SCYL2 reduced the number of virus particles released from cells infected with wild-type HIV-1, but not a strain lacking vpu, in a BST2-dependent manner. SCYL2 stimulated the dephosphorylation of Vpu on Ser52 and Ser 56 by recruiting protein phosphatase 2A (PP2A) to Vpu. Conversely, depletion of SCYL2 resulted in enhanced phosphorylation of Vpu and increased viral particle release. Moreover, SCYL2 was produced in response to type I IFN and contributed to IFN-mediated viral restriction. Together, these results suggest that SCYL2 serves as a regulatory factor for Vpu, reducing the extent of Vpu phosphorylation and consequently enhancing BST2-mediated viral restriction.||Source Title:||Science Signaling||URI:||http://scholarbank.nus.edu.sg/handle/10635/126735||ISSN:||19450877||DOI:||10.1126/scisignal.2003212|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Jul 1, 2020
WEB OF SCIENCETM
checked on Jul 1, 2020
checked on Jun 26, 2020
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.