Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.antiviral.2013.11.012
DC FieldValue
dc.titleInhibition of human cytomegalovirus replication by overexpression of CREB1
dc.contributor.authorChia, Y.L.
dc.contributor.authorNg, C.H.
dc.contributor.authorLashmit, P.
dc.contributor.authorChu, K.L.
dc.contributor.authorLew, Q.J.
dc.contributor.authorHo, J.P.
dc.contributor.authorLim, H.L.
dc.contributor.authorNissom, P.M.
dc.contributor.authorStinski, M.F.
dc.contributor.authorChao, S.-H.
dc.date.accessioned2016-09-06T08:19:12Z
dc.date.available2016-09-06T08:19:12Z
dc.date.issued2014-02
dc.identifier.citationChia, Y.L., Ng, C.H., Lashmit, P., Chu, K.L., Lew, Q.J., Ho, J.P., Lim, H.L., Nissom, P.M., Stinski, M.F., Chao, S.-H. (2014-02). Inhibition of human cytomegalovirus replication by overexpression of CREB1. Antiviral Research 102 (1) : 11-22. ScholarBank@NUS Repository. https://doi.org/10.1016/j.antiviral.2013.11.012
dc.identifier.issn01663542
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/126733
dc.description.abstractExpression of the human cytomegalovirus (HCMV) major immediate-early (MIE) genes is regulated by a strong enhancer-containing promoter with multiple binding sites for various transcription factors, including cyclic AMP response element binding protein 1 (CREB1). Here we show that overexpression of CREB1 potently blocked MIE transcription and HCMV replication. Surprisingly, CREB1 still exhibited strong inhibition of the MIE promoter when all five CREB binding sites within the enhancer were mutated, suggesting that CREB1 regulated the MIE gene expression indirectly. Promoter deletion analysis and site-directed mutagenesis identified the region between -130 and -50 upstream of the transcription start site of the MIE gene as the "CREB1 responsive region". Mutations of SP1/3 and NF-κB binding sites within this region interrupted the inhibitory effect induced by CREB1 overexpression. Our findings suggest that overexpression of CREB1 can cause repression of HCMV replication and may contribute to the development of new anti-HCMV strategies. © 2013 Elsevier B.V. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.antiviral.2013.11.012
dc.sourceScopus
dc.subjectCREB1
dc.subjectHuman cytomegalovirus
dc.subjectMajor immediate-early genes
dc.subjectNF-κB
dc.subjectSP1
dc.subjectSP3
dc.typeArticle
dc.contributor.departmentMICROBIOLOGY
dc.description.doi10.1016/j.antiviral.2013.11.012
dc.description.sourcetitleAntiviral Research
dc.description.volume102
dc.description.issue1
dc.description.page11-22
dc.description.codenARSRD
dc.identifier.isiut000331485900002
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

8
checked on Aug 14, 2019

WEB OF SCIENCETM
Citations

7
checked on Aug 14, 2019

Page view(s)

21
checked on Aug 16, 2019

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.