Please use this identifier to cite or link to this item: https://doi.org/10.1039/c2lc20696j
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dc.titleMicro-patterned porous substrates for cell-based assays
dc.contributor.authorEvenou, F.
dc.contributor.authorDi Meglio, J.-M.
dc.contributor.authorLadoux, B.
dc.contributor.authorHersen, P.
dc.date.accessioned2016-09-06T05:44:36Z
dc.date.available2016-09-06T05:44:36Z
dc.date.issued2012-05-07
dc.identifier.citationEvenou, F., Di Meglio, J.-M., Ladoux, B., Hersen, P. (2012-05-07). Micro-patterned porous substrates for cell-based assays. Lab on a Chip - Miniaturisation for Chemistry and Biology 12 (9) : 1717-1722. ScholarBank@NUS Repository. https://doi.org/10.1039/c2lc20696j
dc.identifier.issn14730197
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/126673
dc.description.abstractIn the search for new therapeutic chemicals, lab-on-a-chip systems have recently emerged as innovative and efficient tools for cell-based assays and high throughput screening. Here, we describe a novel, versatile and simple device for cell-based assays at the bench-top. We created spatial variations of porosity on the surface of a membrane filter by microcontact printing with a biocompatible polymer (PDMS). We called such systems Micro-Printed Membranes (μPM). Active compounds dispensed on the porous areas, where the membrane pores are not clogged by the polymer, can cross the membrane and reach cells growing on the opposite side. Only cells immediately below those porous areas could be stimulated by chemicals. We performed proof-of-principle experiments using Hoechst nuclear staining, calcein-AM cell viability assay and destabilization of the cytoskeleton organisation by cytochalasin B. Resulting fluorescent staining properly matched the drops positioning and no cross-contaminations were observed between adjacent tests. This well-less cell-based screening system is highly flexible by design and it enables multiple compounds to be tested on the same cell tissue. Only low sample volumes in the microlitre range are required. Moreover, chemicals can be delivered sequentially and removed at any time while cells can be monitored in real time. This allows the design of complex, sequential and combinatorial drug assays. μPMs appear as ideal systems for cell-based assays. We anticipate that this lab-on-chip device will be adapted for both manual and automated high content screening experiments. © 2012 The Royal Society of Chemistry.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1039/c2lc20696j
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentMECHANOBIOLOGY INSTITUTE
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1039/c2lc20696j
dc.description.sourcetitleLab on a Chip - Miniaturisation for Chemistry and Biology
dc.description.volume12
dc.description.issue9
dc.description.page1717-1722
dc.description.codenLCAHA
dc.identifier.isiut000302368200021
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