Please use this identifier to cite or link to this item: https://doi.org/10.7150/ijbs.6357
Title: Loop nucleotide polymorphism in a putative miRNA precursor associated with seed length in rice (Oryza sativa L.)
Authors: Wang, C.
Ye, J. 
Tang, W.
Liu, Z.
Zhu, C.
Wang, M.
Wan, J.
Keywords: miRNA
Polymorphism
Rice
Seed length
Issue Date: 2013
Citation: Wang, C., Ye, J., Tang, W., Liu, Z., Zhu, C., Wang, M., Wan, J. (2013). Loop nucleotide polymorphism in a putative miRNA precursor associated with seed length in rice (Oryza sativa L.). International Journal of Biological Sciences 9 (6) : 578-586. ScholarBank@NUS Repository. https://doi.org/10.7150/ijbs.6357
Abstract: The terminal loop region of primary miRNA transcripts is an important determinant controlling miRNA function in human, animals and plants. However, the effects of polymorphisms in loop region of miRNA precusors on phenotypic variation have not been reported, especially on agronomic traits in rice. From rice japonica variety Koshihikari and indica Guichao2, we isolated a precursor of putative miRNA osa-MIR2923a. We detected a GG/AA polymorphism in the loop structure from japonica and indica rice varieties, which was corresponding to japonica/indica rice differentiation. By using high-resolution melting (HRM) analysis, we measured the polymorphisms in a RIL (recombinant inbred lines) population derived from japonica variety Koshihikari and indica Guichao2. We found that the GG/AA polymorphism in the osa-MIR2923a loop was correlated to grain length and length-width ratio. We further found the significant association between seed length and GG/AA polymorphism in a population consisting of 72 rice landraces. Three targets were predicted, whose expressions showed significant differences between the two varieties. Our results suggested that the putative miRNA precursor and the three target genes could play functional roles for indica/japonica seed differentiation. © Ivyspring International Publisher.
Source Title: International Journal of Biological Sciences
URI: http://scholarbank.nus.edu.sg/handle/10635/126617
ISSN: 14492288
DOI: 10.7150/ijbs.6357
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