Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.bbrc.2012.01.032
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dc.titleTransgene insertion in intronic sequences of Mdga2 gene shows methylation in an imprinted manner in an Acrodysplasia (Adp) mouse line
dc.contributor.authorSuzuki, M.
dc.contributor.authorSolter, D.
dc.contributor.authorWatanabe, T.
dc.date.accessioned2016-09-06T03:01:25Z
dc.date.available2016-09-06T03:01:25Z
dc.date.issued2012-02-17
dc.identifier.citationSuzuki, M., Solter, D., Watanabe, T. (2012-02-17). Transgene insertion in intronic sequences of Mdga2 gene shows methylation in an imprinted manner in an Acrodysplasia (Adp) mouse line. Biochemical and Biophysical Research Communications 418 (3) : 439-444. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bbrc.2012.01.032
dc.identifier.issn0006291X
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/126566
dc.description.abstractThe Acrodysplasia (Adp) mutation arises from the insertion of a transgene containing a mouse metallothionein-promoted bovine papilloma virus and human growth hormone-releasing factor gene. Although the transgene is not expressed, mice that are hemizygous for the transgene show skull and paw deformities when the progeny receive the transgene paternally. To elucidate the molecular mechanisms underlying the mutant phenotype and the modified transmission pattern of the Adp phenotype, a junctional fragment around the transgene integration site was cloned. The transgene was inserted into the intronic sequences between exon 3 and exon 4 of the Mdga2 gene and the degree of methylation of the transgene and the severity of the phenotype were reciprocally related in that the transgene was highly or under methylated in normal and deformed mice, respectively. Thus, methylation of the transgene appears to regulate phenotypic expression and imprinting of Adp. © 2012 Elsevier Inc.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.bbrc.2012.01.032
dc.sourceScopus
dc.subjectGenomic imprinting
dc.subjectInsertional mutation
dc.subjectLimb development
dc.subjectMdga2
dc.subjectMethylation
dc.typeArticle
dc.contributor.departmentDUKE-NUS GRADUATE MEDICAL SCHOOL S'PORE
dc.description.doi10.1016/j.bbrc.2012.01.032
dc.description.sourcetitleBiochemical and Biophysical Research Communications
dc.description.volume418
dc.description.issue3
dc.description.page439-444
dc.description.codenBBRCA
dc.identifier.isiut000300925300001
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