Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.neures.2012.11.006
Title: Probing the function of neuronal populations: Combining micromirror-based optogenetic photostimulation with voltage-sensitive dye imaging
Authors: Tsuda, S. 
Kee, M.Z.L. 
Cunha, C.
Kim, J. 
Yan, P.
Loew, L.M.
Augustine, G.J.
Keywords: Cerebellum
Channelrhodopsin
Digital micromirror device
Inhibitory circuitry
Optogenetics
Voltage-sensitive dye imaging
Issue Date: Jan-2013
Citation: Tsuda, S., Kee, M.Z.L., Cunha, C., Kim, J., Yan, P., Loew, L.M., Augustine, G.J. (2013-01). Probing the function of neuronal populations: Combining micromirror-based optogenetic photostimulation with voltage-sensitive dye imaging. Neuroscience Research 75 (1) : 76-81. ScholarBank@NUS Repository. https://doi.org/10.1016/j.neures.2012.11.006
Abstract: Recent advances in our understanding of brain function have come from using light to either control or image neuronal activity. Here we describe an approach that combines both techniques: a micromirror array is used to photostimulate populations of presynaptic neurons expressing channelrhodopsin-2, while a red-shifted voltage-sensitive dye allows optical detection of resulting postsynaptic activity. Such technology allowed us to control the activity of cerebellar interneurons while simultaneously recording inhibitory responses in multiple Purkinje neurons, their postsynaptic targets. This approach should substantially accelerate our understanding of information processing by populations of neurons within brain circuits. © 2013 Elsevier Ireland Ltd and the Japan Neuroscience Society.
Source Title: Neuroscience Research
URI: http://scholarbank.nus.edu.sg/handle/10635/126539
ISSN: 01680102
DOI: 10.1016/j.neures.2012.11.006
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